竹叶纤维树脂吸附分离茶多酚的研究综述.doc

刘晓平:E-mail:584081584@ 竹叶纤维树脂吸附法制备茶多酚的研究 朱兴一，刘晓平，谢捷，王平 （浙江工业大学 药学院，绿色制药技术与装备教育部重点实验室，浙江 杭州 310032） 摘要：研究了利用竹叶纤维树脂吸附绿茶提取液制备低咖啡碱(CAF)含量的茶多酚(TP)工艺。本文首次报道应用逆流提取技术和竹叶纤维吸附树脂法对茶多酚进行提取、吸附分离。树脂柱经水洗除杂质，40%乙醇洗产品，并运用高效液相色谱筛选工艺参数，最后经冷冻干燥制得茶多酚产品。实验结果表明，竹叶纤维对茶多酚及表没食子儿茶素没食子酸酯（EGCG)具有很强的吸附力而对咖啡碱吸附能力差，优化后的工艺参数为：室温，TP、EGCG、CAF静态吸附量分别为117.81mg/g、53.1mg/g、9mg/g；洗脱条件为水2BV，40%醇2BV。茶多酚产品TP、EGCG、CAF含量分别达到98%、48%、0.31%，得率10.18%。本工艺纯绿色，低成本，高得率，适合茶多酚的工业化生产。 关键词：茶多酚；竹叶纤维；逆流提取；吸附 Study on adsorption and preparation of tea polyphenol with bamboo leaf fiber ZHU Xing-yi, LIU Xiao-ping, XIE Jie, WANG Ping (College Of Pharmaceutical Science, Zhejiang University Of Technology. Key Laboratory for Green Pharmaceutical Technologies and Related Equipment, Ministry of Education, Hangzhou 310014, China) Abstract:To study the adsorption and preparation of low-caffeine(CAF) tea polyphenol(TP) with bamboo leaf fiber resin(BLFR). Extracting and separation of TP by countercurrent extraction technology and BLFR was reported firstly. After adsorbing tea extracts, the resin column was eluted by water and 40% ethanol.We then use high performance liquid chromatography(HPLC) to filter optimal parameters and get final product using spray drying method. The results show that BFLR has a strong adsorption ability to TP and [(-)-Epigallocatechingallate] (EGCG) but weak to CAF. The optimal conditions are as follows: room temperature, with amount of static adsorption to TP, EGCG, CAF 117.81mg/g, 53.1mg/g, 9mg/g respectively; eluting condition with 2BV water and 2BV 40% ethanol. Contents of TP, EGCG, CAF in product reached 98%, 48%, 0.31% . The process is green, economic, high yield and might be benefit for industry. Key words: Tea polyphenol; countercurrent extraction; Bamboo fiber; adsorption —————————————— 收稿日期： *通讯联系人 基金项目：国家自然科学基金 茶多酚是茶叶中所含多羟基酚类化合物的总称，约占茶叶干重的15%-25%。研究表明，茶多酚具有很强的抗氧化性能和清除自由基的能力，在临床上具有抗辐射、抗衰老、抗消炎与过敏、抑菌杀菌、抑制癌细胞增长[1-4]等作用，因此从茶叶中提取茶多酚具有十分重要的作用。但茶叶中同时含有1.5%-5%的咖