Optimization of human interferon gamma production in Escherichia by response surface methodology.pdfVIP

Biotechnology and Bioprocess Engineering 2008, 13: 7-13 DOI/10.1007/s12257-007-0126-5 Optimization of Human Interferon Gamma Production in Escherichia coli by Response Surface Methodology = sá?í?ê=bK=_~??éê~?=eéê????éòI=iìò=jK=qK=m~ò=j~????~??I=b?á?á?=jé?á?~=oá?éê?I=^?~=mK=_~ê?~= ?é=?~=o??~I=ié~??ê?=dK=lê??￥éò=^?é?é??I=~??=^?í??á?=aé=iéμ?=o??ê∞?ìéòG=                       _ !## $ %     (!)*  %  $%$ $ _+ ^??íê~?í= The production of human interferon gamma (hIFN-γ) using a synthetic gene in b??üéêá?üá~=???á BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, bio- mass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a sec- ond order response function was developed. The optimal expression conditions were a temperature of 32.6oC, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized con- ditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in bK=???áK ? KSBB héó??ê??W=Escherichia coli _iONJpf, ?á?á?ì?=?é?áì?, íüéê~ééìíá?=éê?íéá?, ?ó?íüéíá?=?é?é= = = = = fkqolar`qflk= 4  .   +4      9):$ ? . .  . .   4  41 ,     -./01γ2   3