水稻根尖铁的积累及附着形态研究.PDFVIP

中国生态农业学报 2009 年 9 月 第 17 卷 第 5 期 Chinese Journal of Eco-Agriculture, Sept. 2009, 17(5): 929?932 DOI: 10. 3724/SP.J.1011.2009.00929 水稻根尖铁的积累及附着形态研究* 章 艺 1, 2 刘 鹏 2** 宋金敏 2 徐根娣 2 蔡妙珍 2 崔洁晨 2 (1. 衢州学院 衢州 324000; 2. 浙江师范大学植物学实验室 金华 321004) 摘 要 以铁毒耐性水稻(Oryza sativa L.)品种“9308”为材料, 利用火焰原子吸收光谱法和化学浸提法研究 0~10 mm 段水稻根尖铁的积累及附着形态。结果表明: 高 Fe2+(400 μmol·L?1)胁迫下, 0~2 mm 段水稻根尖铁 含量最高; 随着 Fe2+浓度的升高, 根边缘细胞铁含量也明显增大; 初生根上不同形态铁的含量为铁锰氧化物 态残渣态有机态可交换态碳酸盐态。以上结果表明, 水稻根尖 0~2 mm 段是过量铁的主要作用位点, 铁的 附着形态以紧密结合态为主, 包围在外的根边缘细胞能固定过量铁。 关键词 铁 水稻 根尖 根边缘细胞 铁形态 中图分类号: S435.1 文献标识码: A 文章编号: 1671-3990(2009)05-0929-04 Forms and accumulation of iron at rice root tip ZHANG Yi1,2, LIU Peng2, SONG Jin-Min2, XU Gen-Di2, CAI Miao-Zhen2, CUI Jie-Chen2 (1. Quzhou College, Quzhou 324000, China; 2. Key Laboratory for Botany, Zhejiang Normal University, Jinhua 321004, China) Abstract To explore the forms and accumulation of iron at root tips in rice (Oryza sativa L.) under Fe2+ toxicity stress, iron-tolerant rice variety “9308” was germinated under laboratory conditions and the seedlings were treated with various Fe2+ con- centrations (0~400 μmol·L?1). The content and forms of iron at 0~10 mm root tips were measured via Flame Atomic Absorption Spectrometric and sequential extraction. Under 400 μmol·L?1 Fe2+ stress, iron content in 0~2 mm root tip is highest, while that in root border cells increases with increasing Fe2+ concentration. The order of contents of various forms of iron in primary root is Fe-Mn oxide combined form residual fo