氧化亚铁硫杆菌对电子垃圾焚烧迹地重金属形态的影响-环境工程学报.doc

拟再审修改稿 氧化亚铁硫杆菌对电子垃圾焚烧迹地重金属形态的影响 赵国华1,4 ，高顺芝2 ，罗兴章3* （ 1.嘉兴学院，生物与化学工程学院，嘉兴 314001；2.苏州中石化，苏州215007；3.复旦大学环境科学与工程系，上海200433；4.嘉兴市化工清洁工艺重点实验室，嘉兴 314001) 摘要：采用生物淋滤法处理电子垃圾焚烧迹地重金属严重污染的土壤. 所用氧化亚铁硫杆菌是从矿坑废水中通过一系列培养、分离、纯化得到.实验结果表明生物淋滤法可以有效地去除土壤中重金属Cu、Pb和Zn，去除率的大小顺序为ZnCuPb；采用五步连续提取法分析处理前后土壤中重金属的存在形态，结果表明通过氧化亚铁硫杆菌处理受重金属污染的土壤，可以促使易移动的重金属结合态的溶解(可交换态、碳酸盐结合态和Fe-Mn氧化物结合态)，并使难移动的重金属结合态向易移动的重金属结合态转变. 关键词：氧化亚铁硫杆菌；电子垃圾；重金属；生物浸出 中图分类号：X53 文献标示码A文章编号： Partitioning variation of heavy metals in contaminated soil by E－waste open burning activities via Thiobacillus Ferrooxidans bioleaching Zhao Guohua1，4，Gao Shunzhi2，Luo Xingzhang3* （1.College of Biological and Chemical Engineering, Jiaxing University, Jiaxing 314001, China；2. Sinopec Group ,Suzhou 215007, China 3.Department of Environmental Science and Engineering; Fudan University; Shanghai 200433;4.Key Laboratory of Clean Chemical Process of Jiaxing, Jiaxing 314001, China） Abstract: Metal removal by biological solubilization in strongly contaminated soil by E－waste open burning activities was carried out in shake flask experiments. Thiobacillus ferrooxidans isolated from coal mine water was used for bioleaching. The order of average solubilization efficiency of total extractable heavy metals was ZnCuPb, Variations in the binding forms of heavy metals were evaluated using a five-step sequential extraction procedure before and after the bioleaching. The bioleaching process caused prompt solubilization of metals mostly associated with the more labile fractions (exchangeable, carbonated and Fe-Mn oxide bound metals), whereas those associated to the less labile ones (organic / residue fractions) were exchanged towards more labile fractions. The results of this study indicated that the bioleaching process can be useful for efficient removal of heavy metals from soil. Key words:Thiobacillus ferrooxidans (T. f);E-waste; heavy metals; bioleaching 收稿日期 基金项目：嘉兴市科技局（2011AY1043）；嘉兴市化工清洁工艺重点实验室开放基金5）?；嘉兴学院科研启动金项目 作者简介：赵国华（1980～）,博