盐胁迫对不同耐盐性枣树品种根系和叶片中多胺含量及多胺氧.PDFVIP

热带亚热带植物学报　2013， 21(4)： 297~304 Journal of Tropical and Subtropical Botany 盐胁迫对不同耐盐性枣树品种根系和叶片中多胺 含量及多胺氧化酶活性的影响 徐呈祥1,2, 马艳萍1, 徐锡增2* (1. 肇庆学院生命科学学院，广东 肇庆 526061； 2. 南京林业大学森林资源与环境学院，南京 210037) 摘 要 ： 为探讨枣(Ziziphus jujuba Mill.)树中多胺及多胺氧化酶活性与其耐盐性的关系，对耐盐性强的‘金丝小枣’和耐盐性弱的 ‘冬枣’2 年生苗中不同状态和种类的多胺和多胺氧化酶活性进行了研究。结果表明，在 120 mmol L–1 NaCl 盐胁迫下，枣树根 系和叶片中的多胺含量升高，耐盐性强的品种的上升幅度显著大于耐盐性弱的品种；随着盐胁迫时间的延长，根系和叶片中多 胺含量逐渐降低，耐盐性弱的品种的降低幅度显著大于耐盐性强的品种，叶中的多胺含量的降低幅度大于根中的，结合态多胺 的降低幅度大于游离态多胺的、精胺和亚精胺的降低幅度大于腐胺的；两个品种根系和叶片中多胺氧化酶(PAO)活性均明显高 于二胺氧化酶(DAO)活性，盐胁迫下耐盐性弱的品种酶活性增强幅度显著大于耐盐性强的品种。这些说明，枣树品种的耐盐性 与其内源多胺的组成、含量有关，多胺氧化酶的种类与活性对盐胁迫的响应也密切相关，多胺积累有利于提高枣树品种的耐 盐性。 关键词：‘ 金丝小枣’；‘ 冬枣’； 耐盐性； 多胺； 多胺氧化酶； 二胺氧化酶 doi: 10.3969/j.issn.1005–3395.2013.04.002 Polyamine Contents and Polyamine Oxidase Activities in Roots and Leaves of Two Jujube (Ziziphus jujuba Mill.) Cultivars with Contrary Salt Tolerance under Salt Stress XU Cheng-xiang1,2, MA Yan-ping1, XU Xi-zeng2* (1. College of Life sciences, Zhaoqing University, Zhaoqing 526061, China; 2. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China) Abstract: In order to investigate the relationship between salt tolerances of jujube cultivar and polyamine and polyamine oxidase, the polyamine contents and polyamine oxidase activities in two-year-old plants of ‘Jingsixiaozao’ with strong salt tolerance and ‘Dongzao’ with weak salt tolerance were studied. The results showed that under 120 mmol L–1 NaCl salt stress, the polyamine contents in roots and leaves of two jujube cultivars enhanced, and increase degree of ‘Jingsixiaozao’ was significantly larger than that of ‘Dongzao’. As the extension of salt stress days, polyamine contents of roots and leaves decreased, and the decrease degree was in the order as: ‘Dongzao’ ‘Jingsixiaozao’, leaves roots, conjugated polyamines free polyamines, spermine spermidine, respectively. The activities of polyamine oxidases (PAO)