Sodiumtaurocholatecotransporting.PDFVIP

RESEARCH ARTICLE Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus Huan Yan1,2?, Guocai Zhong2?, Guangwei Xu2, Wenhui He2,3, Zhiyi Jing2, Zhenchao Gao1,2, Yi Huang2,3, Yonghe Qi2, Bo Peng2, Haimin Wang2, Liran Fu2,3, Mei Song2,3, Pan Chen2,3, Wenqing Gao2, Bijie Ren2, Yinyan Sun2, Tao Cai2, Xiaofeng Feng2, Jianhua Sui2, Wenhui Li2* 1Graduate program in School of Life Sciences, Peking University, Beijing, China; 2National Institute of Biological Sciences, Beijing, China; 3Graduate program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China Abstract Human hepatitis B virus (HBV) infection and HBV-related diseases remain a major public health problem. Individuals coinfected with its satellite hepatitis D virus (HDV) have more severe disease. Cellular entry of both viruses is mediated by HBV envelope proteins. The pre-S1 domain of the large envelope protein is a key determinant for receptor(s) binding. However, the identity of the receptor(s) is unknown. Here, by using near zero distance photo-cross-linking and tandem affinity purification, we revealed that the receptor-binding region of pre-S1 specifically interacts with sodium taurocholate cotransporting polypeptide (NTCP), a multiple transmembrane transporter