chapter实验室工具研究微生物方法.pptVIP

Tools of the Laboratory（实验）;Main Themes; Over the past 30 years,nearly 20 new infectious（传染性的） agents（介质） have appeared suddenly and unexpectedly（意外的）.;Chapter Overview;Methods of Culturing Microorganisms; If you want to study a specified（特定地） microorganism ,you should do experiments step by setp -------according to the Five I,S.;;;INOCULATION:PRODUCING A CULTURE;ISOLATION:SEPARATE ONE SPECIES FROM ANOTHER;Isolation Technique;Methods for isolating bacteria;The spreading tool;MEDIA:PROVIDING NUTRIENTS IN THE LABORATORY;Types of Media;Physical States of Media;Sample liquid media;Semilolid media;Sample semisolid media;Solid media;Solid media;Agar(琼脂);Nutrient agar（营养琼脂） is a microbiological growth medium commonly used for the routine（常规的） cultivation of non-fastidious（苛求的） bacteria.;Nutrient agar typically contains (w/v): 0.5% peptone （蛋白胨） 0.3% beef extract （牛肉膏） 1.5% agar （琼脂） pH adjusted to neutral at 25 ℃. Nutrient broth does not contain agar.;Peptone （蛋白胨）;Peptone（蛋白胨）;Chemical Content of Media;Natural comounds(天然化合物);;Media;Media to Suit Every Functions ;Basal(general) media;Enriched media(加富培养基);When a media is prepared which have a nutrient constituent（成份） which encourage the growth of particular bacteria or microbe is called enriched media, such as blood agar（血琼脂） and chocolate agar(巧克力琼脂). ;Examples of enriched media;Selective Media(选择性培养基);Comparison of Selective media and general media;Example of selective media;Differential media(鉴别培养基);EMB meida（伊红美蓝琼脂培养基）;Media that differentiate characteristics;A reducing medium contains a substance that absorbs oxygen（氧气） Of slowing the penetration（渗透） of oxygen in a medium.;Carbohydrate fermentation in broths（肉汤）;INCUBATION,INSPECTION,AND IDENTIFECATION;If you incubate in the liquid meida,you may see cloudiness（混浊 ）,sediment （沉淀）and colour. A common manifestation(例证) of growth on solid media is the appearance of colonies（菌落）.;Liquid media;Various conditions of cultures;A mixed culture is a container that hol