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See discussions, stats, and author profiles for this publication at: /publication/7792945 Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection Article in Theriogenology · December 2005 DOI: 10.1016/j.theriogenology.2005.04.003 · Source: PubMed CITATIONS READS 35 73 6 authors, including: Stefan Moisyadi University of Hawai?i at Mānoa 54 PUBLICATIONS 814 CITATIONS SEE PROFILE Pawel Pelczar University of Zurich 46 PUBLICATIONS 1,200 CITATIONS SEE PROFILE Available from: Stefan Moisyadi Retrieved on: 09 August 2016 Theriogenology 64 (2005) 1704–1715 /periodicals/the Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection Takehito Kaneko a,1,3, Stefan Moisyadi a,3, Ryota Suganuma a,2,3, Barbara Hohn b, Ryuzo Yanagimachi a, Pawel Pelczar b,* a Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, HI 96822, USA b Friedrich Miescher-Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland Received 2 March 2005; received in revised form 5 April 2005; accepted 8 April 2005 Abstract The low ef?ciency of current microinjection-based animal transgenesis techniques is largely the result of poor embryo survival. We have developed a new, bacterial recombinase-based transgenesis method. Intracytoplasmic sperm injection (ICSI) of single stranded DNA (ssDNA) complexed with E. coli recombinase RecA into mouse metap