响应面试验优化紫薯皮色素提取工艺及其稳定性分析_海妮_巴音达_阿不都拉_阿巴斯.pdfVIP

56? 2016, Vol.37, No.14 食品科学 ※工艺技术 响应面试验优化紫薯皮色素提取工艺及其 稳定性分析 海妮·巴音达，阿不都拉·阿巴斯* （新疆大学生命科学与技术学院，新疆 乌鲁木齐 830046） 摘? 要：通过Box-Behnken试验设计和响应面分析法，对紫薯皮色素的提取条件进行了优化，并且研究了各种物理 及化学因素对紫薯皮色素稳定性的影响。结果，得出最佳提取条件为：酸醇体积比1∶1（0.5%柠檬酸酸化）、料液 比1∶60（g/mL）、提取温度50 ℃、提取时间20 min。在此优化方案条件下，理论吸光度预测值为0.843，实际吸光 度为0.838。紫薯皮色素的稳定性研究结果显示：日光暴晒条件下光稳定性比较差，自然光和避??条件下，稳定性 良好；4～60 ℃的温度范围内具有非常好的热稳定性；紫薯皮色素在含有K＋、Ca2＋、Na＋、Zn2＋等金属离子的溶液 中表现比较稳定的状态，在含有Mg2＋、Cu2＋、Mn2＋、Fe3＋等金属离子的溶液中产生沉淀；酸性条件有利于紫薯皮 色素的稳定性，而碱性条件不利于紫薯皮色素的稳定性；最大安全使用范围内，紫薯皮色素稳定性几乎不会受到苯 甲酸、山梨酸等一系列常用食品添加剂溶液的影响，可作为食品着色剂。 关键词：紫薯皮；色素；响应面法；稳定性 Extraction and Stability of Purple Sweet Potato Peel Pigment Haini·BAYINDA, Abdulla·ABBAS* (College of Life Science and Technology, Xinjiang University, ürümqi 830046, China) Abstract: Box-Behnken experimental design and response surface methodology were employed to optimize the conditions for extraction of purple sweet potato peel pigment. Also, the effect of various physicochemical factors on the stability of the pigment was addressed. The optimum extraction conditions were obtained as follows: 0.5% citric acid:anhydrous ethanol at a volume ratio of 1:1 as extraction solvent; solid-to-solvent ratio, 1:60 (g/mL); temperature, 50 ℃; and time, 20 min. Under these conditions, the predicted absorbance value of the pigment solution was 0.843 and the experimental value was 0.838. The pigment was unstable under light and strong alkali conditions (pH 7). It was relatively stable at temperatures between 4 and 60 ℃. Metal ions including K+, Ca2+, Na+, Zn2+, Mg2+, Cu2+, Mn2+, and Fe3+ were helpful in the degradation of the pigment