pRetroQ-mCherry-C1逆病毒载体使用说明.pdfVIP

pRetroQ-mCherry-C1 编号 载体名称 北京华越洋 VECT55194 pRetroQ-mCherry-C1 pRetroQ-mCherry-C1 逆病毒载体基本信息： 载体名称: pRetroQ-mCherry-C1 质粒类型: 逆病毒载体；荧光报告载体 高拷贝/低拷贝: 低拷贝 克隆方法: 限制性内切酶，多克隆位点 启动子: CMV IE 载体大小: 7605 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: mCherry(N-端） 载体抗性: 氨苄青霉素 筛选标记: 嘌呤霉素（Puromycin） 克隆菌株: DH5α 宿主细胞（系）: 常规细胞系（293、CV-1、CHO 等），原代细胞 pRetroQ-mCherry-C1 载体是自我失活型逆病毒载体，表达 N 端 mCherry 融合蛋白； 备注: pRetroQ-mCherry-C1 载体能够高效递送目的基因到靶细 胞中，尤其适用于原代细胞和其 它难转染的细胞系。 稳定性: 稳表达 组成型/诱导型: 组成型 病毒/非病毒: 逆转录病毒 pRetroQ-mCherry-C1 载体质粒图谱和多克隆位点信息： pRetroQ-mCherry-C1 载体简介： pRetroQ-mCherry-C1 载体描述 pRetroQ-mCherry-C1 is a high-titer, self-inactivating retroviral vector that facilitates efficient delivery and expression of mCherry, or C-terminal mCherry fusions, to target cells. mCherry is a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein are 587 nm and 610 nm, respectively. The multiple cloning site (MCS) in pRetroQ-mCherry-C1 is located just downstream of the mCherry coding sequence. Genes cloned into the MCS are expressed as C-terminal mCherry fusion proteins when they are in the same reading frame as mCherry and there are no intervening stop codons. The RetroQ retroviral vector backbone incorporates several unique features. This vector contains a puromycin resistance cassette (Puror) driven by the PG