(参考)基于纳米金与碳纳米管_纳米铂_壳聚糖纳米复合物固定癌胚抗原免疫传感器的研究(参考)基于纳米金与碳纳米管_纳米铂_壳聚糖纳米复合物固定癌胚抗原免疫传感器的研究.pdf

2008 年第 66 卷 化 学 学 报 Vol. 66, 2008 第 14 期, 1676～1680 ACTA CHIMICA SINICA No. 14, 1676～1680 ·研究论文· 基于纳米金与碳纳米管-纳米铂-壳聚糖纳米复合物固定癌胚抗原免疫 传感器的研究 闵丽根 袁 若* 柴雅琴 陈时洪 许 阳 付 萍 (西南大学化学化工学院 重庆市现代分析重点实验室 重庆 400715) 摘要 采用纳米金(nano-Au)、多壁碳纳米管-纳米铂-壳聚糖的纳米复合物(MWNT-Pt-CS)及电子媒介体硫堇(Th)固载 抗体制得高灵敏癌胚抗原免疫传感器．首先, 于壳聚糖溶液中用 NaBH4 还原 H2PtCl6, 并将多壁碳纳米管分散于其中制 得碳纳米管-纳米铂-壳聚糖纳米复合物, 并将其滴涂在玻碳电极上成膜; 然后, 吸附电???媒介体硫堇制得硫堇/碳纳米 管-纳米铂-壳聚糖(Th/MWNT-Pt-CS)修饰电极．利用壳聚糖和硫堇分子中大量的氨基固定纳米金并吸附癌胚抗体 (anti-CEA); 最后, 用辣根过氧化物酶(HRP)封闭活性位点从而制得高灵敏电流型免疫传感器．在优化的实验条件下, 该 传感器响应的峰电流值与癌胚抗原(carcinoembryonic antigen)浓度在 0.5～10 和 10～120 ng/mL 的范围内保持良好的线 性关系, 检测限为 0.2 ng/mL． 关键词 免疫传感器; 癌胚抗原; 纳米金; 多壁碳纳米管-纳米铂-壳聚糖复合物 An Immunosensor for Carcinoembryonic Antigen Based on Gold Nanoparticles and Multi-wall Carbon Nanotube-Pt-chitosan Composite MIN, Li-Gen YUAN, Ruo* CHAI, Ya-Qin CHEN, Shi-Hong XU, Yang FU, Ping (Chongqing Key Laboratory of Analytical Chemistry, College of Chemical Engineering, Southwest China University, Chongqing 400715) Abstract A highly sensitive amperometric immunosensor for the determination of carcinoembryonic anti- gen (CEA) was developed. In the solution of nano-Pt doped (Pt-CS), multi-wall carbon nanotubes (MWNT) were ultrasonically dispersed to give a multi-wall carbon nanotube-Pt-chitosan composite (MWNT-Pt-CS). Firstly, this composite was modified on a glassy carbon electrode surface, and then thionine was coated onto it. Subsequently, gold nanoparticles (nano-Au) were adsorbed onto the composite surface through the amino groups of thionine and chitosan for immobilizing CEA antibodies. Fi