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Gene 493 (2012) 267–272 Contents lists available at SciVerse ScienceDirect Gene j ourna l homepage: www.e lsev ie r .com/ locate /geneMicroarray-based identification of conserved microRNAs from Pinellia ternata Tao Xu a,1, Bo Wang a,1, Xuefeng Liu a, Ruijuan Feng a, Miao Dong a, Jishuang Chen a,b,? a College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China b Nanjing University of Technology, ChinaAbbreviations: RT-PCR, reverse transcription polyme Quantitative real-time polymerase chain reaction; P nucleotide mismatch; SSPE, saline–sodium phosphate–e ? Corresponding author at: College of Life Sciences, Hangzhou 310018, China. E-mail address: chenjs@ (J. Chen). 1 These authors contributed equally to this work. 0378-1119/$ – see front matter ? 2011 Elsevier B.V. All doi:10.1016/j.gene.2011.08.009a b s t r a c ta r t i c l e i n f oArticle history: Accepted 25 August 2011 Available online 7 December 2011 Received by A.J. van Wijnen Keywords: MicroRNA Microarray Pinellia ternataA large number of microRNAs (miRNAs) reportedly play important roles in plant development; however, scarcely any of these have been found in Pinellia ternata, a herbaceous plant with special physiologic charac- teristics and important medicinal value. To detect P. ternata miRNAs, an in situ synthesized custom microar- ray of plant miRNAs was employed, following verification of the presence of the miRNAs through reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR (qRT-PCR) in the current study. A total of 54 miRNAs belonging to 23 miRNA families were identified. RT-PCR applied to the eight miRNAs validated the microarray results. qRT-PCR that targeted eleven miRNAs showed the presence of miRNAs in different tissues with different expression levels, especially, miRNA319 expression level in the tubers is near- ly 10 times higher than that in the stalks and leaves. This is the first report on the miRNAs in P. ternata, which will enable further invest