IdentiWcation and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sat.pdfVIP

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IdentiWcation and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sat.pdf

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IdentiWcation and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sat

Theor Appl Genet (2009) 118:1027–1034 DOI 10.1007/s00122-008-0959-0 ORIGINAL PAPER IdentiWcation and mapping of Pi41, a major gene conferring resistance to rice blast in the Oryza sativa subsp. indica reference cultivar, 93-11 Qinzhong Yang · Fei Lin · Ling Wang · Qinghua Pan Received: 12 May 2007 / Accepted: 22 December 2008 / Published online: 20 January 2009 ? Springer-Verlag 2009 Abstract The Oryza sativa subsp. indica reference culti- var (cv.), 93-11 is completely resistant to many Chinese isolates of the rice blast fungus. Resistance segregated in a 3:1 (resistance/susceptible) ratio in an F2 population from the cross between 93-11 and the japonica reference cv. Nipponbare, when challenged with two independent blast isolates. The chromosomal location of this monogenic resistance was mapped to a region of the long arm of chro- mosome 12 by bulk segregant analysis, using 180 evenly distributed SSR markers. Five additional SSR loci and nine newly developed PCR-based markers allowed the target region to be reduced to ca. 1.8 cM, equivalent in Nippon- bare to about 800 kb. In the reference sequence of Nippon- bare, this region includes an NBS-LRR cluster of four genes. The known blast resistance gene Pi-GD-3 also maps in this region, but the 93-11 resistance was distinguishable from Pi-GD-3 on the basis of race speciWcity. We have therefore named the 93-11 resistance Pi41. Seven markers completely linked to Pi41 will facilitate both marker- assisted breeding and gene isolation cloning. Introduction Blast is one of the most destructive diseases of rice world- wide (Ou 1985). The causative agent is the Wlamentous ascomycete Magnaporthe oryzae (Couch and Hohn 2002). Genetic resistance is the most economic, eVective and envi- ronmentally responsible method for its control, but resis- tance genes (R genes) typically lose their eVectiveness after a short period in commercial production. The combining of a spectrum of diVerent R genes through marker-aided selec- tion