1-s2.0-S0956566308003308-main.pdf

S m F E a b c d a A R R A A K M P B A P 1 t o ( V t a a ( ( s a c s s a 0 dBiosensors and Bioelectronics 24 (2008) 773–780 Contents lists available at ScienceDirect Biosensors and Bioelectronics journa l homepage: www.e lsev ier .com/ locate /b ios patially well-defined binary brushes of poly(ethylene glycol)s for icropatterning of active proteins on anti-fouling surfaces .J. Xua,b, H.Z. Li c, J. Li b,c, Y.H. Eric Teod, C.X. Zhud, .T. Kanga,?, K.G. Neoha Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge, Singapore 119260, Singapore Division of Bioengineering, National University of Singapore, 7 Engineering Drive 1, Singapore 117574, Singapore Institute of Materials Research and Engineering (IMRE), 3 Research Link, Singapore 117602, Singapore Department of Electrical and Computer Engineering, National University of Singapore, Kent Ridge, Singapore 119260, Singapore r t i c l e i n f o rticle history: eceived 22 April 2008 eceived in revised form 16 June 2008 ccepted 27 June 2008 vailable online 11 July 2008 a b s t r a c t We report a novel method for micropatterning of active proteins on anti-fouling surfaces via spatially well-defined and dense binary poly(ethylene glycol)s (PEGs) brushes with controllable protein-docking sites. Binary brushes of poly(poly(ethylene glycol) methacrylate-co-poly(ethylene glycol)methyl ether methacrylate), or P(PEGMA-co-PEGMEMA), and poly(poly(ethylene glycol)methyl ether methacrylate), or P(PEGMEMA), were prepared via consecutive surface-initiated atom transfer radical polymerizationseywords: icropatterning rotein iosensor TRP EG (SI-ATRPs) from a resist-micropatterned Si(1 00) wafer surface. The terminal hydroxyl groups on the side chains of PEGMA units in the P(PEGMA-co-PEGMEMA) microdomains were activated directly by 1,1′- carbonyldiimidazole (CDI) for the covalent coupling of human immunoglobulin (IgG) (as a model active protein). The resulting IgG-coupled PEGmicrodomains interact o