Discovery of porcine microRNAs and profiling from skeletal muscle tissues during development.pdfVIP

下载本文档

3
0
约3.83万字
约 8页
2017-04-11 发布于江苏
举报
版权申诉

Discovery of porcine microRNAs and profiling from skeletal muscle tissues during development.pdf

1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Discovery of porcine microRNAs and profiling from skeletal muscle tissues during development

Discovery of Porcine microRNAs and Profiling from Skeletal Muscle Tissues during Development Ting-Hua Huang, Meng-Jin Zhu, Xin-Yun Li, Shu-Hong Zhao* Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, People’s Republic of China Abstract MiRNAs (microRNAs) play critical roles in many important biological processes such as growth and development in mammals. In this study, we identified hundreds of porcine miRNA candidates through in silico prediction and analyzed their expression in developing skeletal muscle using microarray. Microarray screening using RNA samples prepared from a 33-day whole embryo and an extra embryo membrane validated 296 of the predicted candidates. Comparative expression profiling across samples of longissimus muscle collected from 33-day and 65-day post-gestation fetuses, as well as adult pigs, identified 140 differentially expressed miRNAs amongst the age groups investigated. The differentially expressed miRNAs showed seven distinctive types of expression patterns, suggesting possible involvement in certain biological processes. Five of the differentially expressed miRNAs were validated using real-time PCR. In silico analysis of the miRNA-mRNA interaction sites suggested that the potential mRNA targets of the differentially expressed miRNAs may play important roles in muscle growth and development. Citation: Huang T-H, Zhu M-J, Li X-Y, Zhao S-H (2008) Discovery of Porcine microRNAs and Profiling from Skeletal Muscle Tissues during Development. PLoS ONE 3(9): e3225. doi:10.1371/journal.pone.0003225 Editor: Suzannah Rutherford, Fred Hutchinson Cancer Research Center, United States of America Received March 24, 2008; Accepted August 15, 2008; Published September 16, 2008 Copyright: 2008 Huang et al. This is an open-access article distributed under the terms of the Creative Commons A