18-2011-a new wheat-Thinopyrum substitution line(小偃麦代换系).pdf

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18-2011-a new wheat-Thinopyrum substitution line(小偃麦代换系).pdf

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18-2011-a new wheat-Thinopyrum substitution line(小偃麦代换系)

Molecular cytogenetic identification of a new wheat-Thinopyrum substitution line with stripe rust resistance Li-Jun Hu ? Guang-Rong Li ? Zi-Xian Zeng ? Zhi-Jian Chang ? Cheng Liu ? Jian-Ping Zhou ? Zu-Jun Yang Received: 13 March 2010 / Accepted: 5 July 2010 / Published online: 15 July 2010 Springer Science+Business Media B.V. 2010 Abstract A new wheat-Thinopyrum substitution line AS1677, developed from a cross between wheat line ML-13 and wheat-Thinopyrum intermedium ssp. trichophorum partial amphiploid TE-3, was charac- terized by fluorescence in situ hybridization (FISH), sequential Giemsa-C banding, genomic in situ hybridization (GISH), seed storage protein electro- phoresis, molecular marker analysis and disease resistance screening. Sequential Giemsa-C banding and GISH using Pseudoroegneria spicata genomic DNA as probe indicated that a pair of St-chromo- somes with strong terminal bands were introduced into AS1677. FISH using pTa71 as a probe gave strong hybridization signals at the nuclear organiza- tion region and in the distal region of the short arms of the St chromosome. Moreover, FISH using the repetitive sequence pAs1 revealed that a pair of wheat 1D chromosomes was absent in accession AS1677. Seed storage proteins separated by acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulphate-polyacrylamide gel electro- phoresis (SDS) confirmed that AS1677 lacked the gliadin and glutenin bands encoded by Gli-D1 and Glu-D1, further confirming the absence of chromo- some 1D. The introduced St chromosome pair belonging to homoeologous group 1 was identified by newly produced genome specific markers. AS1677 is a new 1St (1D) substitution line. When inoculated with stripe rust and powdery mildew isolates, AS1677 expressed stripe rust resistance possibly derived from its Thinopyrum parent. AS1677 can be used as a donor source for introducing novel disease resistance genes to wheat in breeding programs aided by molecular and cytogenetic markers. Key