V2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing suppressor.pdfVIP

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V2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing suppressor.pdf

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V2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing suppressor

V s J a b a A R R A A K T V R s 1 d e ( i ( B i i s e a e s F s h t o A H 0 dVirus Research 163 (2012) 51–58 Contents lists available at SciVerse ScienceDirect Virus Research journa l homepage: www.e lsev ier .com/ locate /v i rusres 2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing uppressor ie Zhanga,b, Jingyun Donga, Yi Xua, Jianxiang Wua,? State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China Hangzhou Yuhang No. 2 High School, Hangzhou, Zhejiang 311100, PR China r t i c l e i n f o rticle history: eceived 4 December 2010 eceived in revised form 16 August 2011 ccepted 18 August 2011 vailable online 26 August 2011 eywords: a b s t r a c t TheV2proteinofTomatoyellow leaf curl Chinavirus (TYLCCNV)was identifiedasanRNAsilencing suppres- sor by Agrobacterium-mediated co-infiltration. The V2 protein could inhibit local RNA silencing, systemic RNA silencing of the green fluorescent protein (GFP) gene and the spread of a systemic GFP RNA silencing signal. However, theV2 could not interferewith the cell-to-cell spread of RNA silencing. Subcellular local- ization assay indicated that the V2 protein was distributed in the cytoplasm of Nicotiana benthamiana cells, and accumulated in irregular cytoplasmic bodies. The V2 bound 21nt and 24nt small interferingomato yellow leaf curl China virus 2 protein NA silencing suppressor iRNA RNA (siRNA) duplexes and 24nt single-stranded (ss)-siRNA but not 21nt ss-siRNA in electrophoresis mobility shift assays. Expression of the V2 protein via the Potato virus X (PVX) vectors heterogenous system induced severe symptoms in N. benthamiana. In a yeast two-hybrid system, TYLCCNV V2 could interact with itself, but not with SlSGS3, which is known to been involved in RNA silencing pathway and to interact with a closely related Tomato yellow leaf curl virus (TYLCV) V2. These results indicate that lencinTYLCCNV V2 is an RNA si . Introduction Post-transcr