Stromelysin-3 over-expression.pdfVIP

BioMed CentralBMC Cancer ssOpen AcceResearch article Stromelysin-3 over-expression enhances tumourigenesis in MCF-7 and MDA-MB-231 breast cancer cell lines: involvement of the IGF-1 signalling pathway Grit Kasper*1, Matthias Reule2, Miriam Tschirschmann3, Niels Dankert1, Karen Stout-Weider4, Roland Lauster3, Evelin Schrock5, Detlev Mennerich6, Georg N Duda1 and Kerstin E Lehmann7 Address: 1Musculoskeletal Research Center Berlin, Charité – Universit?tsmedizin Berlin, Berlin, Germany, 2Astex Therapeutics, Cambridge, UK, 3University for Technologies, Berlin, Germany, 4Institute of Medical Genetics, Charité – Universit?tsmedizin Berlin, Berlin, Germany, 5Institute of Clinical Genetics, Technische Universit?t, Dresden, Germany, 6Boehringer Ingelheim Pharma GmbH CoKG, Biberach, Germany and 7Center for Cardiovascular Research, Charité – Universit?tsmedizin Berlin, Berlin, Germany Email: Grit Kasper* - Grit.Kasper@charite.de; Matthias Reule - Matthias.Reule@; Miriam Tschirschmann - tschirschmann@drfz.de; Niels Dankert - NielsKD@gmx.de; Karen Stout-Weider - karen.stout@charite.de; Roland Lauster - lauster@drfz.de; Evelin Schrock - Evelin.Schrock@mailbox.tu-dresden.de; Detlev Mennerich - detlevmennerich@gmx.de; Georg N Duda - georg.duda@charite.de; Kerstin E Lehmann - K.Lehmann@charite.de * Corresponding author Abstract Background: Stromelysin-3 (ST-3) is over-expressed in the majority of human carcinomas including breast carcinoma. Due to its known effect in promoting tumour formation, but its impeding effect on metastasis, a dual role of ST-3 in tumour progression, depending on the cellular grade of dedifferentiation, was hypothesized. Methods: The present study was designed to investigate the influence of ST-3 in vivo and in vitro on the oestrogen- dependent, non-invasive MCF-7 breast carcinoma cell line as well as on the oestrogen-independent, invasive MDA-MB- 231 breast carcinoma cell line. Therefore an orthotopic human xenograft tumour model in nude mice,