1290_Infinity_customerpres_April30handout.pdfVIP

1Agilent 1290 Infinity LC June 06, 2005Page 1 Version: April 30, 2009 Agenda ? UHPLC: Situation Challenges ? 1290 Infinity LC System ? Performance Examples ? Overview ? 1290 Infinity Technologies ? 1290 Infinity Pump ? 1290 Infinity Autosampler ? 1290 Infinity FlexCube ? 1290 Infinity Thermostatted Column Compartment ? 1290 Infinity DAD ? 1290 Infinity LC for LC/MS ? Method Transferability ? Summary Page 2 2Month ##, 200XPage 3 What Debate ? Why is it Over ? 3Short History of UHPLC 2003 Agilent 1200 Binary 1.8um RRHT Columns 2004 Waters Acquity 1.7um BEH Columns 2006 Agilent 1200 RRLC Thermo Accela Jasco Xtreme-LC 2007 Shimadzu UFLCxr Hitachi LaChrom Ultra Scient. Syst. Ultra HP 2008 Dionex RSLC Knauer Platin Blue 2009 PE Flexar 10 New UHPLC Systems in 5 Years – All different. Page 5 10 New UHPLC Systems in 5 Years – All different UHPLC – Situation 2009 10 New UHPLC Systems in 5 Years – All different. Differencies: Problems: 10 New UHPLC Systems in 5 Years – All different Debate ? System Volumes ? Pressures ? Flow Rates ? Temperatures ? Data rates ? Sensitivity ? Injection ? Method Transfer - Not possible - Requires re-development and re-validation ? Performance Limitations - Speed - Resolution ? Methods: transferable? ? ID ?: 4.6, 3 or 2.1 mm? ? Particle technology: TP or SP? ? Size: STM or larger? principles ? ... ? - Sensitivity - Precision - Linearity Page 6 ? Flow: high or low? ? T: high or low? ? P: 600 or 1000bar? STM = Sub-2um SP = Superficially Porous TP = Totally Porous 41200 UHPLC Situation 2009 – Power Ranges bar 2.1mm ID 3 – 4.6mm ID STM and SP (superficially porous) columnsSTM columns 400 600 800 1000 Vendor A Agilent RRLC Vendor E Vendor C Power = Pressure x Flow Rate 0 200 0 1 2 3 4 5 ml/min Standard LC Vendor D UHPLC Situation 2009 - An “Ideal” System and Its Benefits - Turning “Or” to “And” Ideal: A System that solves all problems ... and ends the UHPLC debate.Characteristics ? Maximum Power Range Benefits ? Seamless Method T