中山大学《细胞生物学》思考题 09.docVIP

  1. 1、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
  2. 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  3. 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
  4. 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
  5. 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们
  6. 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
  7. 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Testing Thinking of Cell Biology 2009.09.28(缺2、3) 1、You are using a cameleon indicator to measure intracellular concentrations of Ca2+.The indicator is composed of a central calmodulin domain, which converts from an extended form to a much more compact form upon calcium binding,and two flanking fluorescent proteins,with CFP attached at one end and YFP attached at the other.You have expressed this indicator in cells and now wish to measure intracellular changes in Ca2+ concentration in response to the biological process you are studying. The instructions say to excite the cameleon at 440 nm and to measure emission at 535 nm.How do you suppose the cameleon indicator works? ◆知识点: ① 报告蛋白 ② CFP(Blue,Cyan)、GFP和YFP ③ 钙调蛋白 ④ 发射光谱(emission spectra) ⑤ 激发光谱(excitation spectra) ◆解题思维(分析能力): ① CFP与YFP的作用 ② Ca2+与钙调蛋白的关系 ◆本题的来源:Miyawaki A, Llopis J, Heim R, McCafferty JM, Adams JA, Ikura M Tsien RY (1997) Fluorescent indicators for Ca2+ based on green fluorescent protein and calmodulin. Nature 388, 882–887. 2009.10.12 1、You are studying the binding of proteins to the cytoplasmic face of cultured neuroblastoma (神经母细胞瘤) cells and have found a method that gives a good yield of inside-out vesicles from the plasma membrane. Unfortunately, your preparations are contaminated with variable amounts of right-side-out vesicles. Nothing you have tried avoids this variable contamination. A friend suggests that you pass your vesicles over an affinity column made of lectin coupled to solid beads. What is the point of your friend’s suggestion? 回答关键词:膜糖仅存在于外膜,凝集素与糖亲和,亲和层析的方法。 2、Gorter and Grendel Revisited. Gorter and Grendels classic conclusion that the plasma membrane of the human erythrocyte consists of a lipid bilayer was based on the following observations:(i) the lipids that they extracted with acetone from 4.74 ×109 erythrocytes formed a monolayer 0.89 m2 in area when spread out on a water surface; and (ii) the surface area of one erythrocyte was about 100μm2, according

文档评论(0)

1243595614 + 关注
实名认证
文档贡献者

文档有任何问题,请私信留言,会第一时间解决。

版权声明书
用户编号:7043023136000000

1亿VIP精品文档

相关文档