爱玉种质资源遗传多样性的ISSR精编.doc

宁德师范学院 毕业设计（论文） 爱玉种质资源遗传多样性的ISSR分析 ISSR Analysis on Genetic Diversity of Ficus pumila var.awkeotsang Germplasm Resources 院 系 ： 生物系 专业（班级）： 2010级生物技术2班 姓 名： 吴泽江 学 号: B2010072205 指导教师： 史辉 职 称： 副教授 完成日期： 2014年 5 月 1 日 宁德师范学院教务处制 摘 要 研究爱玉品种分类、起源进化关系，为育种工作提供理论基础。采用ISSR-PCR分子标记方法对24个品种从100 条ISSR引物中筛选出17条扩增清晰、重复性和多态性好的引物选取4条多态性强的引物扩增结果进行统计和遗传聚类分析4条引物可以将爱玉品种之间遗传差异完全区分开来奠定基础tudy cultivars classification of Ficus pumila var.awkeotsang, and relationship of origin and evolution relationship, which will provide a theoretical basis for breeding work. ISSR-PCR was used to detect the genetic diversity and relationship of 24 Ficus pumila var.awkeotsang cultivars. 17 primers that are amplified clear, good repeatability and polymorphism were screened from100 ISSR primers. And then 4 polymorphic primers were selected from the strongest of amplification, and their amplification results were used to do statistical analysis and analysis of genetic clustering. It was found that the 48 sites were found by 4 primers, and 43 sites showed polymorphism, the percentage of polymorphism loci was 89.6%, and the difference of Ficus pumila var.awkeotsang cultivars can be completely separated by the 4 ISSR primers. 24 Ficus pumila var.awkeotsang cultivars can be divided into four groups by Cluster analysis and principal coordinate analysis, which lays a good foundation for the identification of Ficus pumila var.awkeotsang germplasm. Key words: Ficus pumila var.awkeotsang; ISSR; Genetic diversity; Genetic relationship 目 录 1 引 言 1 1.1 爱玉简介 1 1.2 ISSR在植物遗传多样性研究中的运用 1 1.2.1 品种鉴定和亲缘关系分析 1 1.2.2 物种群体遗传结构和遗传多样性研究 2 1.3 本研究的目的及意义 2 2 材料与方法 3 2.1 材料 3 2.1.1 试材 3 2.1.2 试剂 3 2.1.3 仪器 3 2.2 方法 3 2.2.1 基因组DNA提取和纯度检测 3 2.2.2 ISSR引物的筛选和退火温度优化 4 2.2.3 爱玉群体的ISSR-PCR分析 4 2.2.4 数据分析 4 3 结果与分析 4 3.1 爱玉基因组DNA质量检测 4 3.2 ISSR引物筛选及退火温度优化 5 3.3 爱玉ISSR-PCR遗传分析 6 3.3.1 ISSR引物扩增结果多态性分析 6 3.3.2 爱玉品系遗传多样性分析 8 3.3.3 爱玉品系遗传分化的主坐标分析 9 4 讨 论 11 致 谢 13 参