瓜子金总皂苷的含量测定及其纯化工艺研究Δ.PDFVIP

瓜子金总皂苷的含量测定及其纯化工艺研究Δ 1＊ 2 1 1# 王洪兰 ，张亚楠 ，陈丽红 ，李 祥 （1.南京中医药大学药学院，南京 210046；2.第二军医大学航海与潜水医 学教研室，上海 200433） 中图分类号 R284.2；R282.17 文献标志码 A 文章编号 1001-0408（2014）39-3670-03 DOI 10.6039/j.issn. 1001-0408.2014.39.08 摘 要 目的：建立瓜子金总皂苷含量测定的方法学，研究瓜子金中总皂苷的纯化工艺。方法：采用紫外分光光度法测定瓜子金 中总皂苷含量，考察大孔树脂吸附、富集、纯化瓜子金总皂苷工艺。结果：瓜子金总皂苷在测定范围内均显示较好的线性关系（r ＝ 0.9997）；精密度、重复性、稳定性试验中RSD 值均小于3.00％；加样回收率平均值为99.51％。大孔树脂纯化试验结果表明，70％ 乙醇洗脱部位总皂苷含量达71.53％。结论：本研究所建立的方法快速、准确，可测定瓜子金总皂苷的含量；大孔树脂可有效富集、 纯化总皂苷，为远志属药用植物瓜子金资源的综合利用提供理论支撑。 关键词 瓜子金；总皂苷；含量测定；大孔吸附树脂 Content Determination and Purification Technology of Total Saponins from Polygala japonica 1 2 1 1 WANG Hong-lan ，ZHANG Ya-nan ，CHEN Li-hong ，LI Xiang （1.School of Pharmacy ，Nanjing University of TCM ，Nanjing 210046，China ；2.Dept. of Sailing and Diving Medicine ，Second Military Medical University ， Shanghai 200433，China ） ABSTRACT OBJECTIVE ：To establish a method for the content determination and purification technology of total saponins from Polygala japonica . METHODS ：The content of total saponins from P. japonica was determined by UV visible spectrophotometry. The enrichment and purification of total saponins from P. japonica was determined by macro-porous resin adsorption chromatogra- phy. RESULTS ：The amount of total saponins from P. japonica showed good linear relationship in the range of determination （r ＝ 0.999 7）. RSDs of precision ，repeatability and stability tests were all less than 3.00％. The average recovery rate was 99.51％. The content of total saponins in 70％ethanol elution fraction was 71.53％in macro-porous resin purification test. CONCLUSIONS ：The established method is rapid ，accurate and can be applied for the content determination of total saponins from P. japonica . The en- richment and purification of total sapon