小鼠大脑中动脉栓塞模型的改良及评价【摘要】目的对小鼠大脑中动脉.doc

小鼠大脑中动脉模型的改良及评价 大脑中动脉MCAO）模型C57BL/6J小鼠只测试小鼠远期学习和记忆神经为（2.36±0.21）分相对梗死体积为（25.36±3.65）％MCAO组小鼠潜伏期明显， 【关键词】小鼠；大脑中动脉 Establish and optimize middle cerebral artery occlusion model in mice Tang Ying-xin▲, LIU Min-zhen, DING Feng-fei, ZHANG Qiang, WANG Wei, PAN Deng-ji. ▲Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 【Abstract】Objective: To establish and optimize middle cerebral artery occlusion model in mice. Methods: Fifty C57BL/6J mice were randomly divided into sham group (n=10) and MCAO group (n=40). The mice in MCAO group were used to establish the MCAO model by intraluminal occlusion using monofilament. Twenty-four hours after operation, the nervous function was evaluated and the infarct volume was calculated by TTC staining. Thirty days after operation, Morris water maze test was carried out to investigate spatial learning and memory of mice. Ninety days after operation, head MRI scan was conducted. Results: In MCAO group, the successful rate of execution was 67.5%. The neurological deficit score was (2.36±0.21) and the relative infarct volume was (25.36±3.65)% in MCAO group. Mice in sham group performaced better in water maze task than those in MCAO group. T2-weighted MRI imagines showed infarction clearly. Conclusion: Intraluminal occlusion of MCA using monofilament is a kind of simple and reliable method to establish mouse MCAO model. 【key words】mice; middle cerebral artery occlusion; animal model 大脑中动脉（middle cerebral artery occlusion, MCAO）MCAO模型 1 材料与方法 1.1 材料 1.1.1 实验动物 雄性C57BL/6J小鼠只，体质量20～25 g，分为假手术组MCAO组，由华中科技大学同济医学院实验动物中心提供。 1.1.2 主要试剂与材料 2,3,5-氯化三苯基四氮唑（triphenyltetrazolium chloride, TTC）（购于Sigma公司）；1-FR型动物手术显微镜（购于Zeiss公司）；三洋牌（直径0.128 mm）；硅胶（PROVIL NOVO）（购于Heraeus Kulzer公司）；水合氯醛（购于北京化学试剂公司）磁共振Bruker BioSpin公司）。 1.2 方法 1.2.2 小鼠MCAO模型建立 MCAO模型制备参照ConnollyHata等[]的方法，根据小鼠体型及血管直径的不同而进行改良。10％水合氯醛腹腔麻醉（0.35 mL/100g）小鼠，仰卧于可以加热的护垫上；在手术显微镜下，行颈部正中切口，分离皮下组织，暴露颈动脉鞘，小