Icp超微结构.docVIP

妊娠肝内胆汁淤积症患者胎盘合体滋养细胞 超微结构的定量分析与脐血总胆酸的测定 作者单位：410011 长沙，中南大学湘雅医院妇产科丁依玲 唐玲玲 作者单位：410011 长沙，中南大学湘雅医院妇产科 [摘要]：目的 研究妊娠肝内胆汁淤积症(ICP)胎盘合体滋养细胞形态学改变与胎儿胆酸的变化。方法：分别采用循环酶法测各组胎儿脐静脉血的总胆酸水平；用细胞形态学计量方法分析各组孕妇的胎盘合体滋养层细胞超微结构。结果：（1）ICP组脐静脉血总胆酸水平比正常组显著升高（分别为8.6±3.2，4.6±1.5）（P＜0.05＝。（2）与正常组比较，ICP组胎盘合体滋养细胞微绒毛的数目、数密度（分别为20.6±12.4、1.87±1.3）明显少于正常组（35.4±10.6、4.11±3.2）（P＜0.05＝）；ICP组胎盘合体滋养细胞微绒毛平均体积、表面积密度（分别为0.12±0.005、2.52±0.6）明显大于正常组（分别为0.05±0.001、1.33±0.4）（P0.05）。（3）ICP组胎盘合体滋养细胞线粒体的平均体积，表面积密度, 体积密度（分别为0.018±0.002、0.64±0.01、0.081±0.009）明显大于正常组（分别为0.010±0.005、0.51±0.03、0.053±0.02）（P0.05）。（4）ICP组胎盘合体滋养细胞内质网的平均体积、表面积密度、体积密度（分别为0.023±0.001、0.92±0.01、0.088±0.005）明显大于正常组（分别为0.014±0.003、0.47±0.03、0.052±0.001）（P0.05）。结论：在妊娠肝内胆汁淤积症环境下，胆酸可能损伤胎盘合体滋养细胞的细胞器，影响胎盘的运输和合成功能，可能参与胎儿的损伤。 关键词：胆汁淤积症，妊娠，胆酸，合体滋养细胞，超微结构，体视学 Stereological study on Syncytial cell of human placenta and determinations of total bile acid in cord blood about intrahepatic cholestasis of pregnancy DING Yilin,TANG lingling Department of Gynecoloy and Obstetrics, XiangYa Second Hospital,Central South University, Changsha, 410011 Objective: Our present work was conducted to investigate the relevance of the alterations of fetal bile acid and morphology of ultrastructure on the human placental Syncytial cell, together with fetal bad prognosis about intrahepatic cholestasis of pregnancy. Methods：Total bile acid of cord veinal blood was investigated by cycle enzyme method between intrahepatic cholestasis of pregnancy and control groups after birth. The ultrastructure of human placental Syncytial cell was analyzed by the method of cell morphology measure in two groups. Results: Our study shows that:(1) The total bile acid of cord veinal blood in ICP group was significantly higher than in control group（p0.05）.(2)The analytic outcome of norphometry is following.The amount and number density of syncytial microvilli ultrastructure reduced significantly in ICP group,compared with control group（p0.05）.The average volum and surface area density of p