DNA VACCINES University of Pittsburgh核酸疫苗匹兹堡大学.pptVIP

CONCLUSION DNA vaccines are in their early phase. There are no DNA vaccines in market at present. But this just the beginning . DNA vaccines are going to be the vaccines of next generation. References Immunology by Kuby 6th Edition Immunology by Tizard 4th Edition THANK YOU Queries ? NEERAJ KUMAR ,AVTAR , (students) JITENDER MEHLA (Research Scholar) ,NDRI and Dr. S.K. Sood , Senior scientist,NDRI,Karnal CONTENTS Introduction History DNA vaccines Vs Traditional vaccines How DNA vaccine is made Methods of delivery How DNA vaccine works Advantages Disadvantages Current clinical trials Safety issues Future of DNA vaccines Conclusion References INTRODUCTION DNA vaccine is DNA sequence used as a vaccine. This DNA Sequence code for antigenic protein of pathogen. As this DNA inserted into cells it is translated to form antigenic protein. As this protein is foreign to cells , so immune response raised against this protein. In this way ,DNA vaccine provide immunity against that pathogen. HISTORY In 1990, University of Wisconsin, Jon Wolff found that injection of DNA plasmids produce a protein response in mice. In 1993, Merck Research Laboratories, Dr. Margaret Liu found that intramuscular injection of DNA from influenzae virus into mice produced complete immune response In 1996, trials involving T-cell lymphoma, influenzae herpes simplex virus were started DNA vaccines Vs Traditional vaccines Uses only the DNA from infectious organisms. Avoid the risk of using actual infectious organism. Provide both Humoral Cell mediated immunity Refrigeration is not required Uses weakened or killed form of infectious organism. Create possible risk of the vaccine being fatal. Provide primarily Humoral immunity Usually requires Refrigeration. DNA vaccines Traditional vaccines HOW DNA VACCINE IS MADE Viral gene Expression plasmid Plasmid with foreign gene Recombinant DNA Te