DNA Extraction from Wheat Germ SchoolwiresDNA提取小麦胚芽 schoolwires.pptVIP

下载本文档

2
0
约2.45千字
约 21页
2017-02-13 发布于上海
举报
版权申诉

DNA Extraction from Wheat Germ SchoolwiresDNA提取小麦胚芽 schoolwires.ppt

1、本文档共21页，可阅读全部内容。
2、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

DNA Extraction from Strawberries Lab Safety and Tips Wear Goggles Important to have clean glassware. Follow directions CAREFULLY or you will not get good results. I DNA Single molecules of DNA are long and stringy. Each cell of your body contains six feet of DNA, but its only one-millionth of an inch wide. Chromosomes Strands of DNA molecules coated with protein Start with a Strawberry: look at lab background Easy to pulverize- Why? Already has enzymes-Which ones? Large genome (lots of chromosomes)-what is octoploid? Add Detergent Detergent/shampoo captures protein and releases DNA Mashing Strawberries in a thick Ziploc bag Careful!! You don’t want Bubbles! DNA clings to the bubbles. ? Salt The salt in the extraction buffer keeps the DNA molecules together and separates them from the protein. Filtering the mixture will remove cell debris and organelles. 95% Cold Alcohol Alcohol separates the DNA from the other components of the cell Note: slowly pouring down the side of the test tube Good Tip Be careful to pour down the side of the container to form a layer on top instead of mixing the two layers. ETHANOL MUST BE COLD! The DNA will rise into the alcohol layer and can be ‘spooled’ onto the wooden stick be carefully swirling the stick at the interface of the two layers. DNA will look like snot White Stringy You are viewing the DNA and protein When DNA extractions are performed, you can expect three basic results. No DNA –you messed up! DNA appears fluffy – you sheared in the extraction process DNA appears as thin threads. (You Rock!) Does you DNA look like this? If not…Possible Errors Impurities could have easily been introduced during any step, which may have altered the outcome. Disturbing the solution as the DNA precipitated could cause poor results probably due to the strands of DNA (breaking up). Not allowing enough time for each step. You can stain the chromosomes A. make a wet mount of a thin smear of the chromosomes B. Place one drop of Methy