八角茴香中莽草酸提取工艺研究.docVIP

广 西 科 技 大 学（筹） 毕 业 论 文 课题名称 八角茴香中莽草酸提取工艺研究 英文名称 Investigation on the extraction process of shikimic acid from star anise 姓 名 袁小强 学 号 200800606036 指导教师 蒋世云 系 别 生物与化学工程学院 班 级 生物工程081班 二○一二年五月十八日 摘要 莽草酸，有抗炎、镇痛作用，也是合成目前在临床上唯一一种有效抗禽流感药物磷酸奥斯米韦(商品名：哒啡)的关键原料。莽草酸在八角属植物中含量较高，而以八角茴香中的含量最高。在全球禽流感事件屡次发生的情况下，莽草酸的需求量不断扩大，良好的工艺对莽草酸的扩大再生产意义重大，也对人类社会的发展有着一定的推进作用。 本论文将研究八角茴香中莽草酸的提取工艺，运用回流提取法，较为系统的研究八角茴香果实中提取莽草酸的因素：提取温度、提取时间、料液比，以确定提取的最佳工艺。所得结果为：回流提取法的最优条件为提取时间90min，提取温度80℃，料液比1:30，提取率达到10.78%。本实验对工业上的扩大生产，具有一定的借鉴意义。 关键词：八角茴香、莽草酸、回流提取法、最优工艺 Abstract Shikimic acid has the effect of analgesia and antiphlogistic. It is also a key material for chemical synthesis of oseltamivir phosphate (Tamiflu) which is considered the only effective anti-bird flu drug in clinic. Shikimic acid highly exists in Illicium plants; star anise is the highest content one. At the situation that global avian influenza events happen frequently, the need of shikimic acid expands rapidly, a good technology is meaningful to shikimic acid’s expand production, it also has some positive effect to the development of human society. In this paper, the extraction process of shikimic acid from Illicium plants were investigated using reflux extraction method, which the factors of extracting shikimic acid from Illicium plants systematically were studied in order to get the best exteaction process: extraction temperature, extraction time and solid-liquid ratio. The result showed that reflux extraction method’s best condition is extraction time at 90 minutes, extraction temperature at 80℃, solid-liquid ratio at 1:30, the highest extraction rate is 10.78%. This experiment has some significance to the expand production in practical industy. Key words: Star anise, Shikimic acid, Reflux extraction, Optimum process