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降钙素基因相关肽在银屑病斑块型皮损的分泌与表达降钙素基因相关肽在银屑病斑块型皮损的分泌与表达临床皮肤科杂志 2000年第4期第29卷 临床及实验研究作者：何焱玲　丁桂凤　候凌飞　邓玉兰　王宪　朱铁君　范少光单位：朱铁君 何焱玲（北京医科大学人民医院皮肤科，北京100044）；邓玉兰 丁桂凤（北京医科大学免疫学系，北京100083）；王宪 候凌飞（北京医科大学心血管研究所，北京100083）；范少光（北京医科大学生理学系，北京100083）关键词：银屑病；神经肽；降钙素基因相关肽摘要：为了解精神心理刺激诱发加重银屑病的原因，探讨神经肽在银屑病发病机理中的作用。研究了降钙素基因相关肽(CGRP)在银屑病皮损中的分泌与表达，并确定CGRP作用的靶细胞。应用特异性放射免疫反相法分析，测定32例寻常型银屑病慢性斑块型皮损组织提取液中CGRP的含量，用抗生物素蛋白?生物素?过氧化物酶复合物法和兔抗人CGRP抗体进行免疫组化染色，观察CGRP分泌表达的部位和作用的靶细胞。结果寻常型银屑病慢性斑块型皮损组织提取物中CGRP的含量高于正常人，经统计学分析有显著性差异(P＜0.01)。同时免疫组化染色形态学观察发现CGRP分泌表达于皮损真皮毛细血管壁周及内皮细胞表面，以及表皮细胞间和树枝状细胞表面。提示寻常型银屑病斑块型皮损局部神经肽CGRP的分泌增加，皮损真皮微血管内皮细胞和表皮树枝状细胞与神经肽CGRP密切接触。CGRP与银屑病局部病变密切相关。中图分类号：R758.63文献标识码：A文章编号：1000-4963(2000)04Expression and secretion of calcitonin gene related peptide in psoriatic plaque lesionsHE Yan ling,ZHU Tie jun（Department of Dermatology, Peoples Hospital, Beijing Medical University, Beijing 100044， China）DING Gui feng（Department of Immunology, Beijing Medical University, Beijing 100083, China）HOU Ling fei, WANG Xian（Institute of Cardiovascular Disease, Beijing MedicalUniversity, Beijing 100083, China）DENG Yi lan, FAN Shao?guang（Department of Physiology, Beijing Medical University, Beijing 100083,China)Abstract: In order to study the mechanism of the effect of stress on psoriasis and the involvement of neuropeptides in psoriatic pathogenesis, we investigated the expression and secretion of calcitonin gene related peptide(CGRP) in psoriatic plaquelesions, then identified the target cells of CGRP in psoriatic plaque lesions. Specific radioimmunoassay(RIA) was used to determine CGRP secretive content and immuno histochemistry staining with biodin avidin peroxidase compound method to observe the target cells of CGRP in psoriatic plaque lesion tissue of 32 cases of psoriasis vulgaris. The results showed that the content of CGRP in psoriatic plague lesions was higher than that of normal controls(P＜ 0.01).CGRP was also found on periphly and the endothelial cells of the dermal capillaries and on the surface of epiderma