【2017年整理】大鼠耳科常规手术径路.docxVIP

PAGE \* MERGEFORMAT 6 大鼠耳科常规手术径路 李鹏1,2 丁大连1,2,3 高可雷2,3 Richard Salvi 2,3 1中山大学附属第三医院耳鼻咽喉-头颈外科 2 Center for Hearing and Deafness, University at Buffalo 3中南大学湘雅医院耳鼻咽喉头颈外科 第一作者：李鹏 通讯作者：丁大连 E-mail: HYPERLINK mailto:dding@buffalo.edu dding@buffalo.edu 课题资助：1、National Institute for Occupational Safety and Health： R01 OH010235, 2、广东省科技计划项目：2011B031800359 3、国家外国专家局审批类项目：CG2013440003 摘要 目的: 介绍几种在大鼠耳科实验研究中常用的手术方法。方法: 将大鼠麻醉后，根据不同的实验需求，分别演示经耳后切口的后鼓室径路、经耳上切口的上鼓室径路、经颈部腹侧切口的下鼓室径路、经颈部背侧切口的枕后径路等手术方法。结果：通过不同的手术径路可从不同的角度暴露大鼠的中耳和内耳，为引导耳蜗生物电反应的各种耳蜗电极置放、经圆窗膜转染或置药、药物经耳蜗外淋巴腔灌流、耳蜗内淋巴腔灌注、听骨链中断、以及内淋巴囊手术破坏等各种不同实验目的提供最佳手术方案。结论：根据大鼠颞骨解剖特点，选择不同的中耳内耳手术径路，可以为实现不同的实验目的服务，对实验成功提供技术保障。 关键词: 大鼠, 中耳, 内耳, 手术径路 Standard surgical approaches of the ear surgery in rats Peng Li1,2 Dalian Ding1,2,3 Kelei Gao2,3 Richard Salvi 2,3 1 The Third Affiliated Hospital of Sun Yat-Sen University 2 Center for Hearing and Deafness, University at Buffalo 3Xiangya Hospital, Central South Universit Abstract Object：To introduce several otologic surgical approaches for specific experimental studies in rats. Methods: Based on the specific goals of the experiment, anesthetized rats were prepared for the ear surgery using different surgical approaches aimed at optimizing the experimental outcome. These surgical approaches included: posterior tympanum, superior tympanum, inferior tympanum, and occipital surgeries. Result: The middle ear cavity and the inner ear can be exposed from different angles by using different surgical approaches. For example, electrode placement for recording of cochlear bioelectric responses can be achieved using approaches through the posterior tympanum or inferior tympanum. Alternatively, drug delivery or gene transfection via round window membrane can be most easily accomplished using a surgical approach from the posterior tympanum. Cochlear perfusion of protective or ototoxic drug can be best performed using the inferior tympanum approach. Oss