metabo的lomics2010_xia.pptVIP

Group 30 and 45 are well seperated, 0 group overlap with group 15 * * The parameter panel on top is for a list of compounds The parameter panel on the bottom is for concentration table * Feature selection - SAM METABOLOMICS 2010 * Data Download METABOLOMICS 2010 * METABOLOMICS 2010 * Updates Forecast Recently upgraded Support for multiple group analysis One-way ANOVA post-hoc analysis To be added To add some advanced methods for Association analysis / ROC / OPLS To enhance web interfacing with XCMS Allow local installation To be released by the end of this summer METABOLOMICS 2010 * Data Interpretation Manual approach Background knowledge plus literature search Basic Intuitive Can be very accurate Issues Time-consuming Subjective Lack of statistical strength * METABOLOMICS 2010 Introducing MSEA http://www.msea.ca Metabolite Set Enrichment Analysis Identify biological meaningful patterns from quantitative metabolomics data METABOLOMICS 2010 * Biologically meaningful Associated to the same biological process (i.e. pathway) Associated with the same genetic traits (i.e. SNP) Co-regulated in the same pathological conditions (i.e. disease) Located in the same cellular compartment Enriched in certain tissues/ organs METABOLOMICS 2010 * The MSEA approach METABOLOMICS 2010 * Assess metabolite set directly (GlobalTest) Compound concentrations Compound selection (t-tests, ANOVA, PLS-DA) Abnormal compounds Compound concentrations Metabolite set libraries Over-representation Analysis Quantitative Enrichment Analysis Biological interpretation Find enriched biological themes Compound concentrations Important compound lists Single Sample Profiling Compare to normal references MSEA @ www.msea.ca * METABOLOMICS 2010 Over-representation analysis METABOLOMICS 2010 * Compound label standardization METABOLOMICS 2010 * Identify abnormal concentration METABOLOMICS 2010 * Library selection METABOLOMICS 2010 * Result METABOLOMICS 2010 * Download METABOLOMICS 2010 * MSEA summar