人参单体Rh2对肺腺癌耐药细胞促凋亡作用的研究人参单体Rh2对肺腺癌耐药细胞促凋亡作用的研究.doc

人参单体Rh2对肺腺癌耐药细胞促凋亡作用的研究 胡　硕1,胡成平1,梁昌华1,黄柏英2 (1.中南大学湘雅医院,湖南长沙410008;　2.中南大学细胞培养中心,湖南长沙410008) 〔基金项目〕湖南省科技厅科研基金课题(03SSY3054)。 〔作者简介〕胡　硕(1971-),女,湖南郴州人,医学博士,主治医师,主要从事肿瘤核医学研究。 出处：湖南中医学院学报 2005年4月第25卷第2期 〔摘要〕目的探讨人参单体Rh2对肺腺癌耐药A549 DDP细胞的促凋亡作用。方法用MTT法分别检测顺铂和Rh2对A549细胞、A549 DDP细胞的抑制率(IC)。将Rh2作用于A549 DDP细胞,Hoechst33258染色荧光显微镜下观察细胞核形态,流式细胞仪检测细胞跨膜电位(Δψ)。结果顺铂对A549细胞、A549 DDP细胞的IC50分别为24μmol/L、325μmol/L,耐药指数为13.54。Rh210μmol/L时,对A549 DDP细胞有明显抑制作用,且与Rh2的浓度有一定的依赖关系。Rh2作用后A549 DDP细胞核缩小或变形,荧光成团块分布,有凋亡小体形成;细胞线粒体跨膜电位显著性降低(P0.01)。结论Rh2能促使A549 DDP细胞凋亡,其可能是通过降低细胞的线粒体跨膜电位来诱导细胞凋亡。 〔关键词〕人参单体Rh2;凋亡;肺腺癌细胞;细胞跨膜电位;耐药 〔中图分类号〕R 285.5　〔文献标识码〕A　〔文章编号〕1000—5633(2005)02—0014—03 The Effects of Rensenoside Rh2 on Lung Adenocarcinoma Cells Hu Shuo, Hu Chengping, Liang Changhua, et al (Dept.of NuclearMedicine, Xiangya Hospital, Central SouthUniversity, Changsha 410008) 　　〔Abstract〕ObjectiveTo explore the effects of Gensenoside Rh2 on lung adenocarcinoma cells.Meth-odsThe method ofMTTwas applied to test the resistance of cisplatin and Gensenoside Rh2 to A549 cellsand A549DDP cells respectively. Cellular morphology was observed under fluorescence microscope. Mem-brane potential ofmitochondrion (Δψ) was inspectedwith flowcytometry.ResultsIC50of cisplatine toA549cells and to A549DDP cells were 24μmol/L and 325μmol/L respectively. When Rh210μmol/L,A549cells and A549DDP cells were restrained obviously. Observed under fluorescence microscope, fluorescencewas distributed uniformly onA549DD cell nucleuswithout treatment.After treated byRh2, fluorescencewasconglomerated like grain on the cell nucleus.Many cell nucleuswere shrunkor distorted.Δψof cells treatedby Rh2 were markedly lower than that without treatment,P0.01.ConclusionsGensenoside Rh2 can cause A549DDP cells apoptosis by reducingΔψof cells. 〔Key words〕Gensenoside Rh2; Apoptosis; Lung adenocarcinoma cells;Δψ; Resistance 　　化疗是治疗晚期肺癌患者的重要手段,临床上化疗失败的主要原因是肺癌细胞对抗癌药物产生耐药。寻找既能有效克服肿瘤细胞的耐药,毒副作用又小的抗癌药物,是目前临床上急需解决的问题。传统的中草药中不少药