我国六城市性病高危人群生殖支原体感染状况的分子流行病学研究.x..docx

我国六城市性病高危人群生殖支原体感染状况的分子流行病学研究我国六城市性病高危人群生殖支原体感染状况的分子流行病学研究中华皮肤科杂志 1999年第3期第32卷 论著作者：骆　丹　梁国钧　胡春梅　黄澍杰　谢瑞亭　孙郎炎　朱文元　徐文严单位：骆丹　朱文元　谢瑞亭　210029南京医科大学第一附属医院皮肤科；梁国钧　徐文严　中国医学科学院　中国协和医科大学皮肤病研究所；胡春梅　广东省东莞市慢性病防治院；黄澍杰　广东省江门市皮肤病防治所；孙郎炎　常州市人民医院皮肤科关键词：生殖支原体感染流行病学【摘要】目的　对我国部分地区不同类型的性病人群进行生殖支原体(Mg)感染状况检测分析，以明确Mg感染在非淋菌性尿道炎中的可能致病作用。方法采用两对引物的聚合酶链反应技术，根据Mg粘附蛋白基因及支原体种属内核糖体基因序列设计引物，收集广东、昆明、上海、南京、常州等地性病门诊和(或)性罪错者的泌尿生殖道分泌物标本。结果性病高危人群的Mg?DNA检出率明显高于正常体检组(χ2=7.822,P＜0.01)，其中广东性病门诊及昆明性罪错人群的Mg?DNA检出率明显高于南京、上海、常州等地两种人群的检出率(χ2=8.536,P%26lt;0.01及χ2=5.885,P%26lt;0.05)。未查到其他相关病原体者亦可查及Mg?DNA；有Mg感染者亦可同时感染其他微生物，如衣原体、解脲支原体等；有尿道炎症状病例中Mg?DNA的检出率高于无尿道炎症状者(χ2=11.667,P%26lt;0.01)。结论性病高危人群中存在生殖支原体的感染，在不同地区不同类型人群组中Mg感染率有一定差异。Molecular Epidemiologic Study of Mycoplasma Genitalium Infection in High Risk Populations of STDs from Six Cities in ChinaLUO Dan, LIANG Guojun, HU Chunmei, et al.Department of Dermatology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029【 Abstract】Objective To detect Mycoplasma genitalium (Mg) infection in high risk populations of STDs from six cities in China and try to clarify the possible role of Mg in the pathogenesis of non? gonococcal urethritis.Methods Polymerase chain reaction (PCR) technique based on 2 pairs of primer for Mg? Pa(adhesion protein) and 16S? rRNA (Mycoplasma genera) was used to detect Mg infection. Urogenital specimens of different high risk populations or urethritis patients of STDs were collected from Guangdong, Kunming, Shanghai, Nanjing, and Changzhou areas.Results The positive detection rate of Mg? DNA in high risk populations of STDs was significantly higher than that of normal group(χ2=7.822, P＜0.01). The positive detection rates in STD clinics from Guangdong and promiscuous persons from Kunming were higher than those detected from Shanghai, Nanjing and Changzhou areas(χ2=8.536, P＜ 0.01 and χ2=5.885,P%26lt; 0.05). Mg? DNA could be detected in those patients without other relevant pathogens. Some patients simultaneously infected both Mg and ot