烟台大学本毕业论文.docVIP

烟台大学本科毕业论文 有机磷阻燃剂与DNA相互作用的光谱法研究 年 级: 2010级 学 号: 201060506222 姓 名: 杨依档 专 业: 海洋渔业科学与技术 指导老师: 李 二零一四年六月 摘 要 脱氧核糖核酸(DNA) 包含所有必需的遗传信息，是控制细胞结构和功能的主要物质。它的两条互补链通过 Watson-Crick 碱基对相连接构成双螺旋分子，其在生命过程中起着非常重要的作用。DNA 是许多有机污染物的主要作用靶，其结构在很多条件下可以被破坏，尤其是和小分子相互作用，所以 DNA 可以用于许多类型疾病的治疗。小分子与 DNA 相互作用的研究已成为生物化学和生命科学中热门的研究课题之一。本论文应用荧光、紫外-可见等光谱技术对有机磷阻燃剂与生物大分子的相互作用进行了研究。选用BDE-47（2，2’，4，4 ’-四溴联苯醚）这种有机磷阻燃剂的主要成分来研究其与DNA的作用方式。从而揭示环境污染物对DNA的影响，呼吁人们减少有机污染物的使用，营造良好的生态环境。 关键词：有机磷阻燃剂；小分子；DNA；紫外吸收光谱；荧光光谱 Abstract Deoxyribonucleic acid (DNA) contains all the genetic information required, is the main material control of cell structure and function. Two complementary strands of double helix molecule on which it is connected via the Watson-Crick base, which plays an important role in the life process. DNA is the main role of many organic pollutants of the target, the structure can be destroyed under many conditions, especially the interactions between small molecules, so DNA can be used to treat many types of diseases. Study on the interaction of small molecules with DNA biological chemistry and life science has become one of the hottest research topics in. The interaction of visible spectroscopy using the fluorescence, UV - on organic phosphorus flame retardant and biomacromolecule was studied. Using BDE-47 (2, 2 , 4, 4 - four polybrominated diphenyl ether) the main component of the organic phosphorus flame retardants to study its interaction with DNA method. To reveal the effects of environmental pollutants on DNA,cal;led on people to reduce the use of organic pollutants, and create a good ecological environment. Keywords: organic phosphorus flame retardant;small molecule;;DNA; ultraviolet absorption spectrum; fluorescence spectrum 目 录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc214816193 摘 要 PAGEREF _Toc214816193 \h IV HYPERLINK \l _Toc214816194 Abstract PAGEREF _Toc214816194 \h V HYPERLINK \l _Toc214816195 第1章 绪 论 PAGEREF _Toc214816195 \h 1 HYPERLINK \l _Toc214816196 1.1 本论