国科大生物信息概要.ppt

T. tengcongensis Complete Genome Analysis The bacteria was designated as Thermoanaerobacter tengcongensis MB4 which was isolated from the hotspring in Tengcong area, Yunnan Province of China, by the scientists working in the institute of microbiology of Chinese Academy of Sciences four years ago. The cell morphology of the bacteria is baculiform, Gram reaction is negative(-), it can not form spore and also can not mobilize. The bacteria is anaerobes to oxygen tolerance, optimum temperature is 75oC, optimum pH 7.0. The genome of T. tengcongensis consists of a single circular chromosome of 2,689,441 base pairs(bp) with an average of 37.6% G+C content. Circular representation of the genome of T. tengcongensis MB4 General feature of the T. tengcongensis MB4 genome General Chromosome size 2,689,445 bp Protein coding region 90.89% Stable RNAs 0.892% G + C ratio 37.6% Predicted protein coding sequences 2802 (average length: 871bp ) Function of strongly homologous gene experimentally demonstrated in another organism 1267 Function proposed based on presence of conserved amino acid motif, structural feature or limited homology 213 Conserved hypothetical protein 639 No homology to any previously reported sequences 687 Stable RNAs Coordinates 16S-23S-5S rRNA operon 53,551~58,234 83,515~88,198 1,485,075~1,489,799 2,151,593~2,156,384 tRNA 20 species , 55 copies Functional annotation of T.tengcongensis Total 2808 proteins 143 proteins belong to protein clusters with multiple functions, thus 320 Others include Blast no hits, Blast hits deduced and multiple clusters Linear representation of the genome of T. tengcongensis MB4 The most similar bacteria with T. tengcongensis MB4 Bacillus halodurans C-125（59.9%, 1681/2808）; Bacillus subtilis （55.8%, 1566/2808）; Thermotoga maritima（48.7%, 1368/2808）; Escherichia coli K-12 MG1655（47.3%, 1327/2808）; Pseudomonas aeruginosa PA01（46.