分子细胞生物学前沿-博士-2010资料.ppt

分子细胞生物学专题----转录延伸因子P-TEFb及其活性调控;Main Points;I. Transcription process;Steps of class II gene transcription;TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH RNA polymerase II (Pol II);RNA polymerase II (Pol II);Pre-initiation;PIC;Pol II;Pol II;Early elongation;Splicing;CycT1;Steps: Pre-initiation Initiation Promoter clearance Elongation Termination ;P-TEFb, its functions and activity regulation;P-TEFb: composition and property ;Physiological functions;;B. Pathological functions;TAR RNA;Trans-cyclin T1 gene causes cardiac hypertrophy;CycT1;LARP7;;P;CycT1;CycT1;How does cell signaling control the disruption of the inactive complex?;Control P-TEFb activity by calcium signaling pathway;UV and HMBA can trigger Ca+2 influx;F1C2 cell;Calcium signaling pathways;F1C2 cell;Calcium signaling pathways;F1C2 cell;Hiding and seeking ….;? isoform of PP1 catalytic subunit involves in mediating the disruption of 7SK snRNP;F1C2 cell;In vivo:PP2B and PP1 work cooperatively to disrupt the inactive complex;In vitro: PP2B and PP1 directly disrupt inactive complex; PP2B:let me work first….;F1C2 cell;How do PP2B and PP1 cooperatively disrupt inactive P-TFb complex?;In vitro….Only PP1 can dephosphorylate pT186 of CDK9;In vitro….PP2B and PP1 work cooperatively to dephosphorylate pT186 of CDK9;CycT1;CycT1;How is liberated P-TFFb Recruited to chromatin;;P;Modified Nuclear Fractionation;Almost all of Brd4 associate with chromatin at basal state;Stimuation induces Brd4 release from chromatin;The release of Brd4 from chromatin is essential from P-TEFb recruitment;The release of Brd4 from chromatin is HDAC-dependent;The release of Brd4 from chromatin is essential from elongation;CycT1;HDAC;Thank you!