膜泡运输的结构基础.ppt

Kartagener综合征又称支气管扩张-鼻旁窦炎-内脏转位综合征 The KIF3 Motor Transports N-cadherin and Organizes the Developing Neuroepithelium Hypertrophy and invading tumor-like rosette phenotype of kap3 cKO mouse KAP3-deficient leads to malignant transformation of neuroepithelium N-cadherin and beta-catenin levels are reduced from cell perphery in cKO brain Impaired Ca2+-dependent cell adhesion in kap3-/- MEFs * c: contral, e:cDHC-/- * Targeted Disruption of Mouse Conventional Kinesin Heavy Chain kif5B, Results in Abnormal Perinuclear Clustering of Mitochondria * * Figure 13-3 Molecular Biology of the Cell (? Garland Science 2008) Intracellular Vesicular Traffic Location of microtubule-associated proteins MAP1A MAP1B MAP2A MAP2B (tau) MAP1A MAP1B MAP2C tau Dendrite Axon Major microtubule-associated proteins The cells transfected with tau type L4 The importance of MAPs for neurite formation Cross-section electron micrographs of MAP-induced processes in Sf9 cells MAP2 MAP2C tau MAP2 in vitro Tau in vitor Organization of MT bundles by MAPs . Spacing of MTs depends on MAPs Insect cell expressing MAP2 Insect cell expressing tau J. Chen et al. Nature. 1992 Cytoskeletal architecture AXON DENDRITE Kinesin and Dynein Microtubule-based transport and intracellular trafficking pathways Hirokawa et al., 2009 Harada et al., JCB, 1998 Golgi vesiculation and lysosome dispersion in cells lacking cytoplasmic dynein Molecular motor - kinesin Structures of N-kinesins, M-kinesins and C-kinesins Phylogenic analysis of all KIFs expressed in mouse/humans, D. melanogaster, C. elegans, and S. cerevisiae. The structure and phylogeny of major mouse kinesins Hirokawa et al., 2009 Q Wang et al., Cell Res. 2010 Principal members of KIFs observed by lowangle rotary shadowing Hirokawa et al., 2005 KIFs bind to cargoes through adaptor or scaffolding protein complexes Hirokawa et al., 2005 Tanaka et al., Cell.93:1174-1158. 1998 KIF3 is functionally diverse family of motor Left/right determination Retinitis pi