1000吨年苏云金芽孢杆菌厂生产工艺初步设计.doc

年产1000吨苏云金芽孢杆菌的发酵车间工艺设计 包文雨 （辽宁石油化工大学，石油化工学院，生物工程1102，辽宁营口，113205021） 1000 tons of Bacillus thuringiensis fermentation process design workshop Bao Wenyu (Class 1102, Department of Biological Engineering, School of Environmental and Biological Engineering, Liaoning Shihua University, Liaoning Yingkou, 1132050215, China) Abstract Bacillus thuringiensis (Bacillus thuringiensis) in 1901, was discovered in Japan in 1911 by the Berliner from the Mediterranean flour moth larvae in the prevalence of isolated and found locations in Germany according to their province and named Bacillus thuringiensis. thuringiensis Called Bacillus thuringiensis, is endogenous Gram-positive Bacillus soil bacteria, initially formed in the spore formation of insecticidal crystal protein (insecticidal crystal protein), insects have specific sensitive rats. The preliminary design of the first Bacillus thuringiensis introduced the development process, and then conducted a discussion of its products, the fermentation of Bacillus thuringiensis There are two process routes, that is, submerged fermentation and solid-state fermentation. They all have their advantages and disadvantages, but after comparison argument, I finally chose the submerged fermentation. Then determined according to the relevant information of Bacillus thuringiensis and production cycle of production, production was carried out in accordance of the material balance and heat balance, the last of the equipment for a reasonable selection Key words: Bacillus thuringiensis; product of; process routes; equipment selection 目 录 摘 要 1 Abstract II 第1章 绪论 1 1.1项目背景 1 1.2苏云金芽孢杆菌介绍 1 1.2.1苏云金芽孢杆菌定义 1 1.2.2苏云金芽孢杆菌资源 2 1.2.3苏云金芽孢杆菌鉴定与分类 3 1.2.4孙云锦芽孢杆菌生态学及安全性 3 1.2.5苏云金芽孢杆菌产品化 4 1.3设计方案 5 1.3.1产品方案 5 1.3.2选址 6 1.3.3车间生产过程设计 6 第2章 工艺流程确定与论证 8 2.1工艺流程确定 8 2.1.1苏云金芽孢杆菌液体深层发酵 8 2.2.2苏云金芽孢杆菌固态发酵 9 2.2.3小结 11 第3章 物料衡算 12 3.1生产条件 12 3.2各设备的物料衡算 12 3.2.1发酵罐物料衡算 12 3.2.2接种罐物料衡算 13 3.2.3过滤后的物料横算 13 3.2.4干燥后的物料衡算 14 第4章 能量衡算 15 4.3 热量衡算基础数据的计算和查取 16