重金属离子的固相萃取和分离技术 毕业论文.docx

PAGE  PAGE 3 重金属离子的固相萃取和分离技术 摘要：以Ni2+、Co2+、Cd2+、Pb2+为模板离子，以PEG-1540为致孔剂，二乙烯三胺为固化剂，与环氧树脂混合均匀后置于60℃烘箱内反应24h，即可制得环氧树脂基离子印迹整体柱。本文基于上述的离子印迹整体柱，对一系列标准浓度的重金属离子溶液进行吸附性测试，从而对整体柱的吸附性能进行研究和评估。针对整体柱合成条件和吸附洗脱条件对整体柱性能的影响，实验的同时也将在不同的条件下对整体柱性能进行测试，对离子印迹整体柱进行优化。 关键词：固相萃取；重金属离子；离子印迹；整体柱 Abstract: Epoxy resin based ion imprinted polymer was prepared by heating a kind of uniformly mixed mixture in an electric oven, whose temperature is kept at 60℃.The mixture that contain epoxy resin takes Ni2+,Co2+,Cd2+ and Pb2+ as template ions, PEG-1540 as pore-creating material and diethylene triamine as curing agent. The present study takes the absorption ability of ion imprinted polymer and its appropriate scale problem as the goal in research is carrying out adsorption experiments in which a series of heavy metal ion standard solutions are adsorbed by ion imprinted polymer. Considered the influence of the monolithic materials synthesis conditions and adsorption conditions，the adsorption properties of the monolithic material will be tested in different conditions in order to optimize the parameters of the ion imprinted polymer. Keywords：solid-phase extraction; heavy metal ions; ion imprinting; monolithic column 目 录 1. 引言……………………………………………………………………….………1 1.1. 背景与研究意义…………………………………………………………….…1 1.2 固相萃取的发展……………………………………………………….……2 1.2.1 固相萃取…………………………………………………………….…..2 1.2.2 分子印迹技术……………………………………………………….…..3 1.2.3 整体柱…………………………………………………….……………..3 2. 实验部分………………………………………………………………….………….5 2.1 实验试剂与仪器………………………………………………………………5 2.1.1 试剂………………………………………..…………………..……….5 2.1.2 实验仪器……………………………………………………………5 2.2 离子印迹整体柱的合成………………………………….……………...……5 2.2.1 称取合成各离子印迹柱所需结晶水化合物的质量………….………5 2.2.2 制备重金属离子印迹整体柱……………………………….…………6 2.3 整体柱处理………………………...………………………………….………6 2.3.1 PEG-1540的去除……………………………………………….…...…6 2.3.2 整体柱中印迹离子的去除…...……………………………….……….7 2.4 重金属离子标准溶液的配制…..……………………………………..………7 2.4.1各离子标准溶液的配制………………………………………………..7 2.4.2 混合标准溶液的配制………………………………………………….7 2.4.3金属离子标注曲线方程测定…………………………………………..8 2.5 整体柱各项性能的