济南大学设计实验水果中VC含量的测定综合实验论文报告方案.docVIP

设计实验 题 目 紫外吸收光谱法测定果蔬中Vc 的含量 学 院 化学化工学院 专 业 应用化学 姓 名 XXX 学 号 XXXXXXXXXX 指导教师 XXX 二O 一二 年 十一月 二十七日 学院 化学化工 专业 应用化学 姓名 XX 学号 XXXXXXXXXXX 实验题目 紫外吸收光谱法测定果蔬中Vc的含量 一、设计实验内容 一、紫外分光光度计确定维生素C的最大吸收波长； 二、配制维生素的标准溶液，在最大吸收波长处测定一系列标准溶液的吸光度并绘制维生素C的标准吸收曲线； 三、捣碎水果及蔬菜组织，用盐酸做维生素C提取剂，离心悬浮液，取上层澄清液并定容，在最大吸光度下用紫外分光光度计测溶液的吸光度，并用碱液处理溶液，求出两者的差值。依据维生素C的标准吸收曲线方程，得出果蔬中维生素C含量。 二、设计实验应完成的工作 1、确定维生素C在紫外分光光度计的最大吸收波长； 2、配制标准溶液并绘制标准曲线； 3、捣碎果蔬可食部分，用盐酸做提取液，配制样品溶液； 4、测定果蔬溶液吸光度并用碱液处理，求两者差值； 学 院 化学化工 专业 应用化学 姓 名 XX 班级 应用化学ＸＸＸ班 学号 XXXXXXX 题 目 紫外吸收光谱法测定果蔬中Vc的含量 指导小组或指导教师评语 评定成绩 20 年 月 日 负责人或指导教师 目 录 摘 要························································································2 Abstract·················································································2 前言··················································································3 1.1 荧光法···············································································4 1.2 2，6-二氯靛酚滴定法（还原型VC）·······································4 1.3　 2，4-二硝基苯肼法·································································5 1.4　 碘量法·················································································5 第二章 实验部分··········································································6 2.1 仪器与试剂 ··········································································6 2.1.1　仪器···················································································7 2.1.2　试剂···················································································6 2.2 实验方法···············································································6 2.2.1　测定波长的选择·············································