WB+淋巴细胞与抗体..ppt

* * * * * * * * * * * * * * * * * * * * 新添加 * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Somatic mutation in V genes During development of B cell, somatic mutations are occurring in the V gene, particularly in the place that codes for the second hypervariable region. Antibody mediated Immune Reactions -Opsonization 调理作用 -Neutralization 中和作用 -IgE and Hypersensitivity 超敏反应 -Antibody-dependent cell-mediated Cytotoxicity ADCC 抗体依赖细胞介导的细胞毒作用 Neutralization of Viruses Antibody-dependent cell-mediated cytotoxicity (ADCC) Opsonization Production of IgE FceR1 Mast cell activation Release of mediators mediators Response of target organs Blood vessels Vascular leakage Bronchi Constriction Intestines Hypermotility IgE and immediate hypersensitivity B mast cell Target cell Killer cell Antibodies – Nature’s pro-drugs Antibody Fc receptor Complement Hybridomas and Monoclonal Antibody 杂交瘤和单克隆抗体 (Georges Kohler and Cesar Milstein, 1975) + Selection in HAT medium Fusion Myeloma B cell Immunization with Antigen A Selection for specific clone Antigen A specific Monoclonal antibody (Hypoxanthine and Thymidine) 抗原表位 1984 Nobel Prize Niels K. Jerne, Georges J. F. Kohler and Cesar Milstein. Genetic engineering antibody Chimeric antibody Humanized antibody Single chain antibody Bispecific antibody Mouse Ab From Mouse Human Ab From Human Chimeric Ab Humanized Ab Mouse Ab Human Ab Single chain antibody Shark VL Camel VH VL Human scFv VH Shark domain antibody (12 kDa) scFv Diabody Triabody Valency Neutralisation Type Application Targets Mode Infliximab Rheumatoid arthritis , Crohns disease, Ulcerative Colitis TNF-α chimeric Basiliximab Acute rejection of kidney transplants IL-2 on activated T cells chimeric Bevacizumab Anti-angiogenic cancer therapy VEGF humanized Abciximab Prevent coagulation in coronary angioplasty receptor GpIIb/IIIa on platelets chimeric Daclizumab Acute rejection of kidney transplants IL-2 on activated T cells humanized Gemtuzumab