NoteontheHeatCoagulationofHomogenatesfromNormal.PDFVIP

Note on the Heat Coagulation of Homogenates from Normal and Precancerous Livers* A. C. Griffin, P h . D . , and C. A. B a u m a n n , P h . D . (From the Department o] Biochemistry, College ol Agriculture, University of Wisconsin,Madison 6, Wisconsin) (Received for publication October 9, 1947) In connection with studies on riboflavin in vari- dilution with water or with homogenates from nor- ous fractions of precancerous livers, the observation mal tissue. Homogenates from normal tissue were was made that homogenates of liver tissue from also diluted with water (Table I). In a more ex- r a t s f e d m -methyl- p - dimethylaminoazobenzene tensive study, various concentrations of the three failed to coagulate at 100~ C., whereas similar azo dyes were fed to a larger group of rats and the homogenates from normal rats coagulated com- animals killed at bi-weekly intervals up to 16 weeks pletely in. 1 to 5 minutes. Accordingly a study and the coagulability of the livers determined as was made of the stability towards heat of homo- before. genates from livers of rats exposed to azo dyes for RESULTS various lengths of time. Homogenates from the livers of rats not fed azo METHODS dyes coagulated completely within 1 to 3 minutes Young adult rats of the Sprague-Dawley strain at the dilution used (10 cc. H20/gm.