Prevention of hepatic oxidative injury by Xiao-Chen-Chi-Tang in mice》.pdfVIP

Journal of Ethnopharmacology 111 (2007) 232–239 Prevention of hepatic oxidative injury by Xiao-Chen-Chi-Tang in mice Sung-Hui Tseng a , Ting-Yi Chien a , Chih-Fu Tzeng a , Yun-Ho Linb , Chih-Hsiung Wu b , Ching-Chiung Wang a,∗ a School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Xing Street, Taipei 110, Taiwan, ROC b School of Medicine, Taipei Medical University, 250 Wu-Xing Street, Taipei 110, Taiwan, ROC Received 19 June 2006; received in revised form 3 November 2006; accepted 18 November 2006 Available online 1 December 2006 Abstract The three purgative Cheng-Chi-Tang decoctions (CCTDs) including Ta-Cheng-Chi-Tang (TCCT), Xiao-Chen-Chi-Tang (XCCT), and Tiao-Wei- Chen-Chi-Tang (TWCCT) are used for treating gastrointestinal disorders, including liver diseases in traditional Chinese medicine. However, the underlying mechanisms as liver disease remedies are far from fully clarified. The objective of the study is to investigate and compare the antioxidant activity of the three purgative CCTDs in order to delineate their hepatic protective potential and mechanism. Antioxidant activity measured with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging test indicated XCCT as the most potent preparation (IC50 8.94 g/ml). In tert- butylhydroperoxide (TBH, 50 mM)-induced lipid peroxidation in ICR mice liver homogenates, XCCT also showed stronger and dose-dependent inhibitory activity against TBH-induced malondialdehyde (MDA, a marker of lipid peroxidation) production (IC50 53.66 g/ml). In addition, XCCT showed dose-dependent protective effect against TBH-induced cytotoxicity in normal human Chung liver