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Parallel microscope-based fluorescence, absorbance and time-of-flight mass spectrometry detection for high performance liquid chromatography and determination of glucosamine in urine》.pdf
Talanta 144 (2015) 275 –282 Contents lists available at ScienceDirect Talanta journal homepage: /locate/talanta Parallel microscope-based fluorescence, absorbance and time-of-flight mass spectrometry detection for high performance liquid chromato- graphy and determination of glucosamine in urine Bo Xiong n, Ling-Ling Wang, Qiong Li, Yu-Ting Nie, Shuang-Shuang Cheng, Hui Zhang, Ren-Qiang Sun, Yu-Jiao Wang, Hong-Bin Zhou Key Laboratory of Pesticides Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China a r t i c l e i n f o a b s t r a c t Article history: A parallel microscope-based laser-induced fluorescence (LIF), ultraviolet–visible absorbance (UV) and Received 15 March 2015 time-of-flight mass spectrometry (TOF-MS) detection for high performance liquid chromatography Received in revised form (HPLC) was achieved and used to determine glucosamine in urines. First, a reliable and convenient LIF 3 June 2015 detection was developed based on an inverted microscope and corresponding modulations. Parallel Accepted 7 June 2015 HPLC-LIF/UV/TOF-MS detection was developed by the combination of preceding Microscope-based LIF Available online 11 June 2015 detection and HPLC coupled with UV and TOF-MS. The proposed setup, due to its parallel scheme, was Keywords:
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