Microscopic and flow cytometric semen assessment of Dutch AI-bucks Effect of semen processing procedures and their correlation to fertility》.pdfVIP

Theriogenology 67 (2007) 863–871 Microscopic and flow cytometric semen assessment of Dutch AI-bucks: Effect of semen processing procedures and their correlation to fertility a,* b a ¨ c K. Peterson , M.A.P.M. Kappen , P.J.F. Ursem , J.O. Nothling , B. Colenbrander a, B.M. Gadella a,d a Department of Farm Animal Health, Utrecht University, P.O. Box 80151, NL-3508 TD Utrecht, The Netherlands b Cryolab, Dijk 30, 5521 AZ Eersel, The Netherlands c Section of Reproduction, Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa d Department Biochemistry and Cell Biology, Utrecht University, P.O. Box 80176, NL-3508 TD Utrecht, The Netherlands Received 15 March 2006; received in revised form 4 November 2006; accepted 5 November 2006 Abstract This study was done to determine the effects of processing techniques on the quality of semen from Dutch AI-bucks with the view on improving pregnancy rates after artificial insemination (AI) with liquid or frozen–thawed semen. Motility of spermatozoa was estimated under a microscope whereas the percentage live spermatozoa and the percentage live spermatozoa with intact acrosomes were determined by means of flow cytometry. Aspects of semen processing that were investigated are storage temperature of liquid semen (i), t