Folate-conjugated Fe3O4 nanoparticles for in vivo tumor labeling》.pdfVIP

Trans. Nonferrous Met. Soc. China 23(2013) 2079−2084 Folate-conjugated Fe O nanoparticles for in vivo tumor labeling 3 4 Chun-jiao ZHOU1 2 1 2 2 2 2 , Shao-hua WANG , Yu ZHOU , Peng-fei RONG , Zi-zi CHEN , Jin-yan LIU , Jian-da ZHOU 1. College of Science, Hunan Agricultural University, Changsha 410128, China; 2. The Third Xiangya Hospital, Central South University, Changsha 410013, China Received 8 March 2012; accepted 10 October 2012 Abstract: Highly biocompatible superparamagnetic Fe O nanoparticles were synthesized by amide of folic acid (FA) ligands and 3 4 the NH -group onto the surface of Fe O nanoparticles. The as-synthesized folate-conjugated Fe O nanoparticles were characterized 2 3 4 3 4 by X-ray diffraction diffractometer, transmission electron microscope, FT-IR spectrometer, vibrating sample magnetometer, and dynamic light scattering instrument. The in vivo labeling effect of folate-conjugated Fe O nanoparticles on the hepatoma cells was 3 4 investigated in tumor-bearing rat. The results demonstrate that the as-prepared nanoparticles have cubic structure of Fe3O4 with a particle size of about 8 nm and hydrated diameter of 25.7 nm at a saturation magnetization of 51 A·m2/kg. These nanoparticles possess good physiological stability, low cytotoxicity