Expression of H5N1 influenza virus hemagglutinin protein fused with protein transduction domain in an alphavirus replicon system》.pdfVIP

Journal of Virological Methods 163 (2010) 31–39 Contents lists available at ScienceDirect Journal of Virological Methods j o u rn al h omepage: w w w. elsev /locate/jviromet Expression of H5N1 influenza virus hemagglutinin protein fused with protein transduction domain in an alphavirus replicon system Shi-gui Yang a,b , Jian-er Wo a , Min-wei Li a , Fen-fang Mi c , Cheng-bo Yu a , Guo-liang Lv a , Hong-Cui Cao a , Hai-feng Lu a , Bao-hong Wang a , Hanping Zhu b , Lan-Juan Li a,∗ a State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China b Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China c Zhejiang Chinese Medical University, Hangzhou 310053, China a b s t r a c t Article history: Alphavirus replicons, in which structural protein genes are replaced by heterologous genes, express Received 24 December 2008 high levels of the heterologous proteins. On the basis of the potencies of replicons to self-replicate Received in revised form 12 July 2009 and express foreign proteins and the remarkable intercellular transport property of VP22, a novel Accepted 21 July 2009 alphavirus Semliki Forest virus (SFV) replicon system of VP22 fused with a model antigen, hemagglutinin Available online 29 July 2009 (HA), of the human-avian H5N1 influenza virus, was explored in this study. Further, replicon parti