Engineering mesenchymal stem cells for regenerative medicine and drug delivery》.pdfVIP

Methods 84 (2015) 3–16 Contents lists available at ScienceDirect Methods journal homepage: www.else /locate/ymeth Engineering mesenchymal stem cells for regenerative medicine and drug delivery Ji Sun Park 1, Smruthi Suryaprakash 1, Yeh-Hsing Lao 1, Kam W. Leong ⇑ Department of Biomedical Engineering, Columbia University, New York, NY 10027, United States a r t i c l e i n f o a b s t r a c t Article history: Researchers have applied mesenchymal stem cells (MSC) to a variety of therapeutic scenarios by harness- Received 28 November 2014 ing their multipotent, regenerative, and immunosuppressive properties with tropisms toward inflamed, Received in revised form 19 February 2015 hypoxic, and cancerous sites. Although MSC-based therapies have been shown to be safe and effective to Accepted 2 March 2015 a certain degree, the efficacy remains low in most cases when MSC are applied alone. To enhance their Available online 11 March 2015 therapeutic efficacy, researchers have equipped MSC with targeted delivery functions using genetic engi- neering, therapeutic agent incorporation, and cell surface modification. MSC can be genetically modified Keywords: virally or non-virally to overexpress therapeutic proteins that complement their innate properties. MSC Mesenchymal stem cells (MSC) can als