《不同毒力株弓形虫速殖子消减cDNA文库的构建》.pdf

2007，23(11) 中国人兽共患病学报 ChineseJOurnalofZOOilOSeS 1127 文章编号：1002—2694(2007)11—1127—04 不同毒力株弓形虫速殖子消减cDNA文库的构建” 张海珠“2，袁保梅。，宋晓荣4，石佑恩 摘要：目的构建不同毒力株弓形虫速殖子eDNA消减文库。方法 以弓形虫强毒株RH株速殖于为Tester、弱毒株 交。分别将获取的2组抑制性消减杂交产物与pGEM 000 98蹦，片段长度在200～2bp之间。结论通过抑制性消减杂交技术成功构建2个消减文库，为进一步筛选和鉴定弓形虫 毒力相关基因奠定了基础。 关键词：弓形虫}虫株；抑制性消减杂交 中图分类号：R382 5文献标识码：A ConstructionofthesubtractivecDNA for librarytachyzoites of indifferentvirulentstains gondii Toxoplasma ZHANGHalzhu，YUANBaomei，SONG Youen Xiao—rong，SHI (DepartmentofParasitology-TongJiMedical College，HtmzhongUniversity oJScienceand 430030，China) Technology，Wuhan ABSTRACT：ToeonstrHctthesubtractivecDNA for of indifferentvirulent 1ibrarytachyzoitesToxoplasmagondii strains． tile subtraetivehubridization(SSH)waswith of RII suppressive the srraina3testerandthoseofthe perforuledtachyzoites Prug— niaudstrainwhhlowvirulenceasthedriver．()nthe reverse contrary，the subtraetive wasclonewith suppressive hybridization testerand ofthe strainas thoseoftheRHstrainasdriver．Theobtainedfrom tachyzoitesPrugniaud products thesetWO hybrid— andthen izationswerelink