5-氨基乙酰丙酸介导的光动力学治疗胃癌的实验研究.pdf

2006;26(3) 南方医科大学学报(J South Med Univ) 255 # # - Effect of photodynamic therapy with 5 aminolevulinic acid on human gastric cancer cells in vitro 1 1 1 1 2 2 2 HUANG Zong-hai , ZHOU Guang-jun , YU Jin-long , LI Zhou , DING Lian-shu , XU Ru-xiang , JIANG Xiao-dan 1 2 Department of General Surgery, Zhuj iang Hospital, Southern Medical University, Guangzhou 510282, China; Department of Neuro- surgery, Zhuj iang Hospital, Southern Medical University, Guangzhou 510282, China Abstract: Objective To investigate the effect of 5-aminolevulinic acid (5-ALA)-mediated photodynamic therapy (PDT) on MGC-803 human gastric cancer cells . MGC-803 human gastric cancer cells were treated with 5-ALA at var- in Uitro Methods ious concentrations followed by laser irradiation. The cells were also treated with 5-ALA at the same concentration before laser exposure at various doses. PDT-induced phototoxicity of the cells was determined by MTT assay . Results After laser exposure of the cells at the same dose (25.0 J/cm2), the cell survival rates decreased significantly with incubation of the cells with 5-ALA at 0.25, 0.5, 1.0, 2.0 and 4.0 mmol/L, respectively (F=266.39, P0.00 1), but 2