间接抑制免疫分析法测定水溶液中痕量磺胺甲 唑.pdfVIP

38 ( FENX IHUAXUE ) 7 2010 7 Ch inese Journ l ofAn lytic l Chem istry 1019~ 1022 DOI: 10. 3724 / SP. J. 1096. 2010. 01019 1 2 1 * 1 唐苹苹 罗昭峰 蔡继宝 苏庆德 1 ( , 230026) 2 ( , 230027) , , , 30 cm, 0. 25 mm 198 nm B IAcore 3000 , , 0. 04~ 10. 0 g/L; 0. 01 g/L, , , , ; ; ; ; 1 [ 1, 2] ( SMX ) , [ 3 ~ 5] , , , SMX , SMX , , BIAcore( SPR ) [ 6 ] , L i SPR SMX [ 7] , ntiSMX (MA bs), SMX , , (M IP)[ 8~ 12] , SMX SMX( SPE) , , 2 2. 1 BIA core 3000 , CM 5( BIAcore AB ); JSM 6700F( JEOL); () ( Su lf th i zole, ST ) ( Su lf di zine, SD ) ( Bovine serum lbum in, BSA ) ( Ethylene glycol dmi eth cryl te, EGDMA ) S igm ; 4( 4V inylpyrid ine, 4VPY, A lf A es r); ( 2, 2 zobi sisobutyron itrile, A IBN ), (); 1. 0 g/L ntiSMX (MAbs) () ( PBS, pH 7. 4, BIA core AB ), 2. 2 SMX [ 15 ] SMX 0. 25 mmol ( No. * Em il: qdsu@ ustc. edu. cn 38 1020 SMX 0. 5 mLN, N , 4VPY, 30 m in, EGDMA2. 5 mL 10 mg A IBN, 30 m in 5 m in, [ 14] ( 30 cm 0. 25 mm i. d) 50 , , 15M P , , 5 m in2 mL ( 41, V/ V), SMX ( N IP), SMX 2. 3 M IPSPESPR SMX BIA core 3000, [ 15], PBS ( pH 7. 4), 25 , 20 L /m in: 100 L 002 g /L ntiSMX MAbs( 1 g /LMAbs PBS( pH 7. 4)50) SMX , 10 L 20 mmol/L N OH ; 100